Production conditions for bruceanol B, an antibiotic that generates oxygen radical

Doseung Lee; Kyung Hwan Boo; Jin Kyu Woo; Gyeong Lyong Jeon; Jin Man Lee; Sang Han Lee; Jae Hoon Kim; Dong Sun Lee; Key Zung Riu

doi:10.3839/jksabc.2011.019

Production conditions for bruceanol B, an antibiotic that generates oxygen radical

Doseung Lee
, Kyung Hwan Boo
, Jin Kyu Woo
, Gyeong Lyong Jeon
, Jin Man Lee
, Sang Han Lee
, Jae Hoon Kim
, Dong Sun Lee
, Key Zung Riu

Research output: Contribution to journal › Article › peer-review

Abstract

Strain DS4, a soil microorganism producing an oxygen radical-generating antibacterial substance, bruceanol B, was isolated, and cultural conditions for the antibiotic production were optimized. Bruceanol B was separated by ethyl acetate extraction, silica gel and Sephadex LH-20 gel column chromatographies, and high-performance liquid chromatography (HPLC). The highest antibacterial activity was obtained from medium containing 1.5% soluble starch and 2.0% yeast extract, cultured at 30°C for 3.5 days.

Original language	English
Pages (from-to)	128-131
Number of pages	4
Journal	Journal of Applied Biological Chemistry
Volume	54
Issue number	1
DOIs	https://doi.org/10.3839/jksabc.2011.019
State	Published - 2011

Keywords

Bruceanol B
Production condition
Radical-generating antibiotic

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10.3839/jksabc.2011.019

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title = "Production conditions for bruceanol B, an antibiotic that generates oxygen radical",

abstract = "Strain DS4, a soil microorganism producing an oxygen radical-generating antibacterial substance, bruceanol B, was isolated, and cultural conditions for the antibiotic production were optimized. Bruceanol B was separated by ethyl acetate extraction, silica gel and Sephadex LH-20 gel column chromatographies, and high-performance liquid chromatography (HPLC). The highest antibacterial activity was obtained from medium containing 1.5\% soluble starch and 2.0\% yeast extract, cultured at 30°C for 3.5 days.",

keywords = "Bruceanol B, Production condition, Radical-generating antibiotic",

author = "Doseung Lee and Boo, \{Kyung Hwan\} and Woo, \{Jin Kyu\} and Jeon, \{Gyeong Lyong\} and Lee, \{Jin Man\} and Lee, \{Sang Han\} and Kim, \{Jae Hoon\} and Lee, \{Dong Sun\} and Riu, \{Key Zung\}",

year = "2011",

doi = "10.3839/jksabc.2011.019",

language = "English",

volume = "54",

pages = "128--131",

journal = "Journal of Applied Biological Chemistry",

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publisher = "Korean Society for Applied Biological Chemistry",

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T1 - Production conditions for bruceanol B, an antibiotic that generates oxygen radical

AU - Lee, Doseung

AU - Boo, Kyung Hwan

AU - Woo, Jin Kyu

AU - Jeon, Gyeong Lyong

AU - Lee, Jin Man

AU - Lee, Sang Han

AU - Kim, Jae Hoon

AU - Lee, Dong Sun

AU - Riu, Key Zung

PY - 2011

Y1 - 2011

N2 - Strain DS4, a soil microorganism producing an oxygen radical-generating antibacterial substance, bruceanol B, was isolated, and cultural conditions for the antibiotic production were optimized. Bruceanol B was separated by ethyl acetate extraction, silica gel and Sephadex LH-20 gel column chromatographies, and high-performance liquid chromatography (HPLC). The highest antibacterial activity was obtained from medium containing 1.5% soluble starch and 2.0% yeast extract, cultured at 30°C for 3.5 days.

AB - Strain DS4, a soil microorganism producing an oxygen radical-generating antibacterial substance, bruceanol B, was isolated, and cultural conditions for the antibiotic production were optimized. Bruceanol B was separated by ethyl acetate extraction, silica gel and Sephadex LH-20 gel column chromatographies, and high-performance liquid chromatography (HPLC). The highest antibacterial activity was obtained from medium containing 1.5% soluble starch and 2.0% yeast extract, cultured at 30°C for 3.5 days.

KW - Bruceanol B

KW - Production condition

KW - Radical-generating antibiotic

UR - https://www.scopus.com/pages/publications/79952730201

U2 - 10.3839/jksabc.2011.019

DO - 10.3839/jksabc.2011.019

M3 - Article

AN - SCOPUS:79952730201

SN - 1976-0442

VL - 54

SP - 128

EP - 131

JO - Journal of Applied Biological Chemistry

JF - Journal of Applied Biological Chemistry

IS - 1

ER -

Production conditions for bruceanol B, an antibiotic that generates oxygen radical

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Keywords

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