Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

Young Ho Hong; Dong Woo Lee; Sang Jae Lee; Eun Ah Choe; Seong Bo Kim; Yoon Hee Lee; Chan Ick Cheigh; Yu Ryang Pyun

doi:10.1007/s10529-006-9277-2

Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

Young Ho Hong, Dong Woo Lee, Sang Jae Lee, Eun Ah Choe, Seong Bo Kim, Yoon Hee Lee, Chan Ick Cheigh, Yu Ryang Pyun

Department of Food and Food Service Inudstry

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t_1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

Original language	English
Pages (from-to)	569-574
Number of pages	6
Journal	Biotechnology Letters
Volume	29
Issue number	4
DOIs	https://doi.org/10.1007/s10529-006-9277-2
State	Published - Apr 2007

Keywords

D-Tagatose
Esherichia coli
Immobilization
L-Arabinose isomerase
Thermotoga neapolitana

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10.1007/s10529-006-9277-2

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title = "Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana",

abstract = "Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.",

keywords = "D-Tagatose, Esherichia coli, Immobilization, L-Arabinose isomerase, Thermotoga neapolitana",

author = "Hong, {Young Ho} and Lee, {Dong Woo} and Lee, {Sang Jae} and Choe, {Eun Ah} and Kim, {Seong Bo} and Lee, {Yoon Hee} and Cheigh, {Chan Ick} and Pyun, {Yu Ryang}",

year = "2007",

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doi = "10.1007/s10529-006-9277-2",

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journal = "Biotechnology Letters",

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T1 - Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

AU - Hong, Young Ho

AU - Lee, Dong Woo

AU - Lee, Sang Jae

AU - Choe, Eun Ah

AU - Kim, Seong Bo

AU - Lee, Yoon Hee

AU - Cheigh, Chan Ick

AU - Pyun, Yu Ryang

PY - 2007/4

Y1 - 2007/4

N2 - Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

AB - Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

KW - D-Tagatose

KW - Esherichia coli

KW - Immobilization

KW - L-Arabinose isomerase

KW - Thermotoga neapolitana

UR - http://www.scopus.com/inward/record.url?scp=33847723004&partnerID=8YFLogxK

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M3 - Article

C2 - 17206372

AN - SCOPUS:33847723004

SN - 0141-5492

VL - 29

SP - 569

EP - 574

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 4

ER -

Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

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Keywords

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