Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

Young Ho Hong; Dong Woo Lee; Sang Jae Lee; Eun Ah Choe; Seong Bo Kim; Yoon Hee Lee; Chan Ick Cheigh; Yu Ryang Pyun

doi:10.1007/s10529-006-9277-2

Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

Young Ho Hong
, Dong Woo Lee
, Sang Jae Lee
, Eun Ah Choe
, Seong Bo Kim
, Yoon Hee Lee
, Chan Ick Cheigh
, Yu Ryang Pyun

Department of Food and Food Service Inudstry

Yonsei University
University of Pennsylvania
Emory University
CJ CheilJedang Corporation
Ohio State University

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t_1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

Original language	English
Pages (from-to)	569-574
Number of pages	6
Journal	Biotechnology Letters
Volume	29
Issue number	4
DOIs	https://doi.org/10.1007/s10529-006-9277-2
State	Published - Apr 2007

Keywords

D-Tagatose
Esherichia coli
Immobilization
L-Arabinose isomerase
Thermotoga neapolitana

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10.1007/s10529-006-9277-2

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title = "Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana",

abstract = "Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.",

keywords = "D-Tagatose, Esherichia coli, Immobilization, L-Arabinose isomerase, Thermotoga neapolitana",

author = "Hong, \{Young Ho\} and Lee, \{Dong Woo\} and Lee, \{Sang Jae\} and Choe, \{Eun Ah\} and Kim, \{Seong Bo\} and Lee, \{Yoon Hee\} and Cheigh, \{Chan Ick\} and Pyun, \{Yu Ryang\}",

year = "2007",

month = apr,

doi = "10.1007/s10529-006-9277-2",

language = "English",

volume = "29",

pages = "569--574",

journal = "Biotechnology Letters",

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TY - JOUR

T1 - Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

AU - Hong, Young Ho

AU - Lee, Dong Woo

AU - Lee, Sang Jae

AU - Choe, Eun Ah

AU - Kim, Seong Bo

AU - Lee, Yoon Hee

AU - Cheigh, Chan Ick

AU - Pyun, Yu Ryang

PY - 2007/4

Y1 - 2007/4

N2 - Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

AB - Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

KW - D-Tagatose

KW - Esherichia coli

KW - Immobilization

KW - L-Arabinose isomerase

KW - Thermotoga neapolitana

UR - https://www.scopus.com/pages/publications/33847723004

U2 - 10.1007/s10529-006-9277-2

DO - 10.1007/s10529-006-9277-2

M3 - Article

C2 - 17206372

AN - SCOPUS:33847723004

SN - 0141-5492

VL - 29

SP - 569

EP - 574

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 4

ER -

Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

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Keywords

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