Protective Role of Peroxiredoxin I in Heat-Killed Staphylococcus Aureus-infected Mice

Hu Nan Sun, Yue Liu, Jian Nan Wang, Chuang Wang, Ren Liu, Ling Zu Kong, Xing Zhen, Nisansala Chandimali, Yu Dong Cui, Sun Uk Kim, Dong Seok Lee, Dae Yeul Yu, Ji Su Kim, Dong Kee Jeong, Taeho Kwon, Ying Hao Han

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Background/Aim: Staphylococcus aureus (S. aureus) is a major gram-positive pathogen, which can cause toxic and immunogenic injuries both in nosocomial and community-acquired infections. Peroxiredoxin (Prx) I plays crucial roles in cellular apoptosis, proliferation, and signal transduction as well as in immunoregulation. The present study aimed to investigate whether Prx I protects mice from death caused by the heat-killed Staphylococcus aureus. Materials and Methods: In the present study, we challenged the wild-type and Prx I-deficient mice with heat-killed S. aureus (HKSA). The effects of Prx I were evaluated by a series of in vitro and in vivo experiments including western blot, Haematoxylin and Eosin staining, splenocyte analysis and cytokines analysis. Results: Intra-peritoneal (ip) inoculation of HKSA resulted in increased mortality of Prx I-knockout (KO) mice with severe liver damage and highly populated spleens with lymphocytes. Furthermore, HKSA infections also bursted the production of both pro-inflammatory and anti-inflammatory serum cytokines in Prx I KO compared to wild-type mice. Conclusion: Enhanced mortality of S. aureus-infected mice with Prx I deficiency suggested that Prx I may protect against the infection-associated lethality of mice.

Original languageEnglish
Pages (from-to)749-755
Number of pages7
JournalIn Vivo
Volume33
Issue number3
DOIs
StatePublished - May 2019

Keywords

  • Inflammatory
  • Intra-peritoneal
  • Knockout
  • Peroxiredoxin I
  • Staphylococcus aureus

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