Protein kinase C signaling molecules regulate encystation of Acanthamoeba.

Eun Kyung Moon; Dong Il Chung; Yeonchul Hong; Hyun Hee Kong

doi:10.1016/j.exppara.2012.07.008

Protein kinase C signaling molecules regulate encystation of Acanthamoeba.

Eun Kyung Moon, Dong Il Chung, Yeonchul Hong, Hyun Hee Kong

Department of Medicine

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Protein kinase C (PKC) is involved in receptor desensitization, membrane biogenesis, transcription regulation, immune response mediation, and cell growth regulation. Results of ESTs analysis and microarray analysis of Acanthamoeba cysts revealed high expression of PKC during encystation of Acanthamoeba. PKC inhibitor, chelerythrine chloride, inhibited cyst maturation of Acanthamoeba. Through domain search analysis, we found 27 types of PKC genes from Acanthamoeba ESTs (AcPKC1-AcPKC27), all of which were defined as atypical PKC isoforms. Results of fluorescence microscopic analysis showed the localization of AcPKC in cell membranes or nuclear membranes during encystation. siRNA against AcPKC reduced the encystation efficiency of Acanthamoeba. These AcPKC may be involved in several signal transduction pathways, especially during encystation of Acanthamoeba.

Original language	English
Pages (from-to)	524-529
Number of pages	6
Journal	Experimental Parasitology
Volume	132
Issue number	4
DOIs	https://doi.org/10.1016/j.exppara.2012.07.008
State	Published - Dec 2012

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title = "Protein kinase C signaling molecules regulate encystation of Acanthamoeba.",

abstract = "Protein kinase C (PKC) is involved in receptor desensitization, membrane biogenesis, transcription regulation, immune response mediation, and cell growth regulation. Results of ESTs analysis and microarray analysis of Acanthamoeba cysts revealed high expression of PKC during encystation of Acanthamoeba. PKC inhibitor, chelerythrine chloride, inhibited cyst maturation of Acanthamoeba. Through domain search analysis, we found 27 types of PKC genes from Acanthamoeba ESTs (AcPKC1-AcPKC27), all of which were defined as atypical PKC isoforms. Results of fluorescence microscopic analysis showed the localization of AcPKC in cell membranes or nuclear membranes during encystation. siRNA against AcPKC reduced the encystation efficiency of Acanthamoeba. These AcPKC may be involved in several signal transduction pathways, especially during encystation of Acanthamoeba.",

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AU - Chung, Dong Il

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AB - Protein kinase C (PKC) is involved in receptor desensitization, membrane biogenesis, transcription regulation, immune response mediation, and cell growth regulation. Results of ESTs analysis and microarray analysis of Acanthamoeba cysts revealed high expression of PKC during encystation of Acanthamoeba. PKC inhibitor, chelerythrine chloride, inhibited cyst maturation of Acanthamoeba. Through domain search analysis, we found 27 types of PKC genes from Acanthamoeba ESTs (AcPKC1-AcPKC27), all of which were defined as atypical PKC isoforms. Results of fluorescence microscopic analysis showed the localization of AcPKC in cell membranes or nuclear membranes during encystation. siRNA against AcPKC reduced the encystation efficiency of Acanthamoeba. These AcPKC may be involved in several signal transduction pathways, especially during encystation of Acanthamoeba.

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Protein kinase C signaling molecules regulate encystation of Acanthamoeba.

Abstract

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