TY - JOUR
T1 - Protein-patterning on functionalized, non-biofouling poly[N-acryloxysuccinimide-co-oligo(ethylene glycol) methyl ether methacrylate] film-coated PET surfaces
AU - Han, Gyeongyeop
AU - Kim, Yoonyoung
AU - Kang, Kyungtae
AU - Lee, Bong Soo
AU - Lee, Jungkyu K.
N1 - Publisher Copyright:
© The Polymer Society of Korea and Springer 2018.
PY - 2018/3
Y1 - 2018/3
N2 - We successfully fabricated poly(ethylene terephthalate) (PET) surfaces through a perfluoroaryl azide-based photochemical reaction, and subsequently formed an intrinsically activated, non-biofouling poly[N-acryloxysuccinimide-co-oligo(ethylene glycol) methyl ether methacrylate] on the surface through surface-initiated, controlled radical polymerization. The grafted copolymer film on PET facilely generated a protein pattern using a microcontact printing technique without employing both an activation step to introduce an active functional group (e.g., succinimidyl ester) and a passivation process for minimizing non-specific adsorption. Consequently, we characterized the functionalized PET surfaces by using various methods including contact angle measurement, X-ray photoelectron spectroscopy (XPS), scanning probe microscopy (SPM), field-emission scanning electron microscopy (FE-SEM). In addition, we evaluated the non-biofouling efficacy of the protein-patterned copolymer film on PET by confocal laser scanning microscopy.
AB - We successfully fabricated poly(ethylene terephthalate) (PET) surfaces through a perfluoroaryl azide-based photochemical reaction, and subsequently formed an intrinsically activated, non-biofouling poly[N-acryloxysuccinimide-co-oligo(ethylene glycol) methyl ether methacrylate] on the surface through surface-initiated, controlled radical polymerization. The grafted copolymer film on PET facilely generated a protein pattern using a microcontact printing technique without employing both an activation step to introduce an active functional group (e.g., succinimidyl ester) and a passivation process for minimizing non-specific adsorption. Consequently, we characterized the functionalized PET surfaces by using various methods including contact angle measurement, X-ray photoelectron spectroscopy (XPS), scanning probe microscopy (SPM), field-emission scanning electron microscopy (FE-SEM). In addition, we evaluated the non-biofouling efficacy of the protein-patterned copolymer film on PET by confocal laser scanning microscopy.
KW - Biopatterning
KW - Copolymer coating
KW - Organic polymer substrate
KW - Photochemical reaction
KW - Surface-initiated controlled radical polymerization
UR - http://www.scopus.com/inward/record.url?scp=85053243545&partnerID=8YFLogxK
U2 - 10.1007/s13233-018-6035-y
DO - 10.1007/s13233-018-6035-y
M3 - Article
AN - SCOPUS:85053243545
SN - 1598-5032
VL - 26
SP - 263
EP - 269
JO - Macromolecular Research
JF - Macromolecular Research
IS - 3
ER -