Proteomic analysis of human macrophages exposed to hypochlorite-oxidized low-density lipoprotein

Jeong Han Kang, Hyun Su Ryu, Hyun Tae Kim, Su Jin Lee, Ung Kyu Choi, Yong Bok Park, Tae Lin Huh, Myung Sook Choi, Tae Cheon Kang, Soo Young Choi, Oh Shin Kwon

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The invasion of monocytes through the endothelial wall of arteries and their transformation from macrophage into form cells has been implicated as a critical initiating event in atherogenesis. Human THP-1 monocytic cells can be induced to differentiate into macrophages by phorbol myristate acetate (PMA) treatment, and can be converted into foam cells by exposure to oxidized low-density lipoprotein (oxLDL). To identify proteins potentially involved in atherosclerotic processes, we performed a proteomic analysis of THP-1 macrophages exposed to oxLDL generated by treatment with native LDL with hypochlorous acid/hypochlorite (HOCl/OCl-). We detected more than a thousand proteins, of which 104 differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) and the NCBI database. The largest differences in expression were observed for bifunctional purine biosynthesis protein, vacuolar protein sorting 33A, breast carcinoma amplified sequence, adenine phosphoribosyltransferase, and tropomyosin alpha 3 chain. Interestingly, many apoptotic proteins such as lamin B1, poly (ADP-ribose) polymerase, Bcl-2 related protein A1 and vimentin were identified by MALDI-TOF analysis. Identities were confirmed by matching the sequence of several tryptic peptides using MALDI-TOF/TOF MS, Western blot analyses and immunofluorescent microscopy. The data described here will contribute to establishing a functional profile of the human macrophage proteome. Furthermore, the proteins identified in this study are attractive candidates for further biomarkers involved in the pathogenesis of atherosclerosis.

Original languageEnglish
Pages (from-to)446-458
Number of pages13
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1794
Issue number3
DOIs
StatePublished - Mar 2009

Keywords

  • Macrophage
  • Oxidized low-density lipoprotein
  • Proteome
  • THP-1 cell
  • Two-dimensional gel electrophoresis

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