TY - JOUR
T1 - Proteomic analysis of outer membrane proteins from Acinetobacter baumannii DU202 in tetracycline stress condition
AU - Yun, Sung Ho
AU - Choi, Chi Won
AU - Park, Soon Ho
AU - Lee, Je Chul
AU - Leem, Sun Hee
AU - Choi, Jong Soon
AU - Kim, Soohyun
AU - Kim, Seung Il
PY - 2008/12
Y1 - 2008/12
N2 - Acinetobacter baumannii readily developed antimicrobial resistance to clinically available antibiotics. A. baumannii DU202 is a multi-drug resistant strain, and is highly resistant to tetracycline (MIC>1,024 μ/ml). The surface proteome of A. baumannii DU202 in response to the sub-minimal inhibitory concentration (subMIC) of tetracycline was analyzed by 2-DE/MS-MS and 1-DE/LC/MS-MS to understand the pathways that form barriers for tetracycline. Membrane expression of major outer membrane proteins (Omps) was significantly decreased in response to the subMIC of tetracycline. These Omps with sizes of 38, 32, 28, and 21 kDa were identified as OmpA38, OmpA32, CarO, and OmpW, respectively. However, transcription level of these Omps was not significantly changed. 1-DE/LC/MS-MS analysis of secreted proteins showed that OmpA38, CarO, OmpW, and other Omps were increasingly secreted at tetracycline condition. This result suggests that A. baumannii actively regulates the membrane expression and the secretion of Omps to overcome antibiotic stress condition.
AB - Acinetobacter baumannii readily developed antimicrobial resistance to clinically available antibiotics. A. baumannii DU202 is a multi-drug resistant strain, and is highly resistant to tetracycline (MIC>1,024 μ/ml). The surface proteome of A. baumannii DU202 in response to the sub-minimal inhibitory concentration (subMIC) of tetracycline was analyzed by 2-DE/MS-MS and 1-DE/LC/MS-MS to understand the pathways that form barriers for tetracycline. Membrane expression of major outer membrane proteins (Omps) was significantly decreased in response to the subMIC of tetracycline. These Omps with sizes of 38, 32, 28, and 21 kDa were identified as OmpA38, OmpA32, CarO, and OmpW, respectively. However, transcription level of these Omps was not significantly changed. 1-DE/LC/MS-MS analysis of secreted proteins showed that OmpA38, CarO, OmpW, and other Omps were increasingly secreted at tetracycline condition. This result suggests that A. baumannii actively regulates the membrane expression and the secretion of Omps to overcome antibiotic stress condition.
KW - Acinetobacter baumannii DU202
KW - Multi-drug resistant (MDR)
KW - Outer membrane proteins (Omps)
UR - http://www.scopus.com/inward/record.url?scp=58149109275&partnerID=8YFLogxK
U2 - 10.1007/s12275-008-0202-3
DO - 10.1007/s12275-008-0202-3
M3 - Article
C2 - 19107403
AN - SCOPUS:58149109275
SN - 1225-8873
VL - 46
SP - 720
EP - 727
JO - Journal of Microbiology
JF - Journal of Microbiology
IS - 6
ER -