Proteomic analysis of phosphotyrosyl proteins in human embryonic stem cell-derived neural stem cells

Jumi Kim; Ji Su Kim; Hye Eun Kim; Young Joo Jeon; Dong Wook Kim; Yunjo Soh; Kang Seok Seo; Hak Kyo Lee; Nag Jin Choi; Hyung Min Chung; Dong Seok Lee; Jung Il Chae

doi:10.1016/j.neulet.2011.05.039

Proteomic analysis of phosphotyrosyl proteins in human embryonic stem cell-derived neural stem cells

Jumi Kim, Ji Su Kim, Hye Eun Kim, Young Joo Jeon, Dong Wook Kim, Yunjo Soh, Kang Seok Seo, Hak Kyo Lee, Nag Jin Choi, Hyung Min Chung, Dong Seok Lee, Jung Il Chae

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Phosphorylation can reveal essential cell functions, such as cell differentiation, signal transduction, metabolic maintenance and cell division. The aim of this study was to investigate phosphorylated protein expression changes during neuronal lineage differentiation from hESCs. To measure the phosphorylated protein expression change during neuronal differentiation, we performed a comparative phosphoproteome analysis using 2-DE after MALDI-TOF MS and an MS/MS protein identification method, making a comparison between neural lineage differentiating cells and normal embryoid bodies (EBs) differentiated from human embryonic stem cells (hESCs) and profiling constituent phosphorylated proteins. Of 36 differentially expressed protein spots, 12 spots were shown to be up-regulated in differentiating neural cells. Specifically, the 7 up-regulated proteins of the 12 have potential roles in neuronal differentiation or neuronal damage recovery, including ACTB, heterogeneous nuclear ribonucleoprotein A2B1 (hnRNP A2B1), heterogeneous nuclear ribonucleoprotein L (hnRNP L), SET, chaperonin-containing TCP-1, vimentin and voltage-dependent anion channel protein 1 (VDAC1). These proteins are discussed further below.

Original language	English
Pages (from-to)	158-163
Number of pages	6
Journal	Neuroscience Letters
Volume	499
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2011.05.039
State	Published - 25 Jul 2011

Keywords

Embryonic body
Human embryonic stem cell
Neural stem cell
Proteomic analysis

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10.1016/j.neulet.2011.05.039

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@article{b667131c78034d56a87cefa51d77063c,

title = "Proteomic analysis of phosphotyrosyl proteins in human embryonic stem cell-derived neural stem cells",

abstract = "Phosphorylation can reveal essential cell functions, such as cell differentiation, signal transduction, metabolic maintenance and cell division. The aim of this study was to investigate phosphorylated protein expression changes during neuronal lineage differentiation from hESCs. To measure the phosphorylated protein expression change during neuronal differentiation, we performed a comparative phosphoproteome analysis using 2-DE after MALDI-TOF MS and an MS/MS protein identification method, making a comparison between neural lineage differentiating cells and normal embryoid bodies (EBs) differentiated from human embryonic stem cells (hESCs) and profiling constituent phosphorylated proteins. Of 36 differentially expressed protein spots, 12 spots were shown to be up-regulated in differentiating neural cells. Specifically, the 7 up-regulated proteins of the 12 have potential roles in neuronal differentiation or neuronal damage recovery, including ACTB, heterogeneous nuclear ribonucleoprotein A2B1 (hnRNP A2B1), heterogeneous nuclear ribonucleoprotein L (hnRNP L), SET, chaperonin-containing TCP-1, vimentin and voltage-dependent anion channel protein 1 (VDAC1). These proteins are discussed further below.",

keywords = "Embryonic body, Human embryonic stem cell, Neural stem cell, Proteomic analysis",

author = "Jumi Kim and Kim, {Ji Su} and Kim, {Hye Eun} and Jeon, {Young Joo} and Kim, {Dong Wook} and Yunjo Soh and Seo, {Kang Seok} and Lee, {Hak Kyo} and Choi, {Nag Jin} and Chung, {Hyung Min} and Lee, {Dong Seok} and Chae, {Jung Il}",

year = "2011",

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doi = "10.1016/j.neulet.2011.05.039",

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journal = "Neuroscience Letters",

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AU - Jeon, Young Joo

AU - Kim, Dong Wook

AU - Soh, Yunjo

AU - Seo, Kang Seok

AU - Lee, Hak Kyo

AU - Choi, Nag Jin

AU - Chung, Hyung Min

AU - Lee, Dong Seok

AU - Chae, Jung Il

PY - 2011/7/25

Y1 - 2011/7/25

N2 - Phosphorylation can reveal essential cell functions, such as cell differentiation, signal transduction, metabolic maintenance and cell division. The aim of this study was to investigate phosphorylated protein expression changes during neuronal lineage differentiation from hESCs. To measure the phosphorylated protein expression change during neuronal differentiation, we performed a comparative phosphoproteome analysis using 2-DE after MALDI-TOF MS and an MS/MS protein identification method, making a comparison between neural lineage differentiating cells and normal embryoid bodies (EBs) differentiated from human embryonic stem cells (hESCs) and profiling constituent phosphorylated proteins. Of 36 differentially expressed protein spots, 12 spots were shown to be up-regulated in differentiating neural cells. Specifically, the 7 up-regulated proteins of the 12 have potential roles in neuronal differentiation or neuronal damage recovery, including ACTB, heterogeneous nuclear ribonucleoprotein A2B1 (hnRNP A2B1), heterogeneous nuclear ribonucleoprotein L (hnRNP L), SET, chaperonin-containing TCP-1, vimentin and voltage-dependent anion channel protein 1 (VDAC1). These proteins are discussed further below.

AB - Phosphorylation can reveal essential cell functions, such as cell differentiation, signal transduction, metabolic maintenance and cell division. The aim of this study was to investigate phosphorylated protein expression changes during neuronal lineage differentiation from hESCs. To measure the phosphorylated protein expression change during neuronal differentiation, we performed a comparative phosphoproteome analysis using 2-DE after MALDI-TOF MS and an MS/MS protein identification method, making a comparison between neural lineage differentiating cells and normal embryoid bodies (EBs) differentiated from human embryonic stem cells (hESCs) and profiling constituent phosphorylated proteins. Of 36 differentially expressed protein spots, 12 spots were shown to be up-regulated in differentiating neural cells. Specifically, the 7 up-regulated proteins of the 12 have potential roles in neuronal differentiation or neuronal damage recovery, including ACTB, heterogeneous nuclear ribonucleoprotein A2B1 (hnRNP A2B1), heterogeneous nuclear ribonucleoprotein L (hnRNP L), SET, chaperonin-containing TCP-1, vimentin and voltage-dependent anion channel protein 1 (VDAC1). These proteins are discussed further below.

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Proteomic analysis of phosphotyrosyl proteins in human embryonic stem cell-derived neural stem cells

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