TY - JOUR
T1 - Proteomic evaluation of adults of Rhyzopertha dominica resistant to phosphine
AU - Park, Byeoung Soo
AU - Lee, Byung Ho
AU - Kim, Tae Wan
AU - Ren, Yong Lin
AU - Lee, Sung Eun
PY - 2008/1
Y1 - 2008/1
N2 - A proteomic study using a PH3-susceptible (RD2) and -resistant strain (CRD343) of Rhyzopertha dominica was undertaken to validate the relation between change of proteins and PH3 resistance. Protein expression levels were compared using PD-Quest program after two-dimensional polyacrylamide gel-electrophoresis. Comparing the intensity of proteins, 15 proteins decreased and 6 proteins were newly expressed in CRD343. After MALDI-TOF and LC-MS/MS analyses, the decreased proteins were identified as arginine kinases, dihydrolipoamide dehydrogenase, Hsp60, reverse transcriptase, glyceraldehydes-3-phosphate dehydrogenase, triosephosphate isomerase and hypothetical proteins. Dihydrolipoamide dehydrogenase is involved in the Krebs cycle and glyceraldehydes-3-phosphate dehydrogenase and triosephosphate isomerase are involved in the glycolysis pathway. Among up-regulated proteins, sodium channel, glutamate racemase, enolase and vitellogenin were identified. Taken together, PH3 affected glycolysis as well as Krebs cycle and the induction of enolase might recover this dysfunction.
AB - A proteomic study using a PH3-susceptible (RD2) and -resistant strain (CRD343) of Rhyzopertha dominica was undertaken to validate the relation between change of proteins and PH3 resistance. Protein expression levels were compared using PD-Quest program after two-dimensional polyacrylamide gel-electrophoresis. Comparing the intensity of proteins, 15 proteins decreased and 6 proteins were newly expressed in CRD343. After MALDI-TOF and LC-MS/MS analyses, the decreased proteins were identified as arginine kinases, dihydrolipoamide dehydrogenase, Hsp60, reverse transcriptase, glyceraldehydes-3-phosphate dehydrogenase, triosephosphate isomerase and hypothetical proteins. Dihydrolipoamide dehydrogenase is involved in the Krebs cycle and glyceraldehydes-3-phosphate dehydrogenase and triosephosphate isomerase are involved in the glycolysis pathway. Among up-regulated proteins, sodium channel, glutamate racemase, enolase and vitellogenin were identified. Taken together, PH3 affected glycolysis as well as Krebs cycle and the induction of enolase might recover this dysfunction.
KW - 2D-PAGE
KW - Dihydrolipoamide dehydrogenase
KW - Enolase
KW - MALDI-TOF
KW - Phosphine resistance
KW - Rhyzopertha dominica
UR - http://www.scopus.com/inward/record.url?scp=36749074621&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2007.10.028
DO - 10.1016/j.etap.2007.10.028
M3 - Article
AN - SCOPUS:36749074621
SN - 1382-6689
VL - 25
SP - 121
EP - 126
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
IS - 1
ER -