Proteomic evaluation of adults of Rhyzopertha dominica resistant to phosphine

Byeoung Soo Park; Byung Ho Lee; Tae Wan Kim; Yong Lin Ren; Sung Eun Lee

doi:10.1016/j.etap.2007.10.028

Proteomic evaluation of adults of Rhyzopertha dominica resistant to phosphine

Byeoung Soo Park
, Byung Ho Lee
, Tae Wan Kim
, Yong Lin Ren
, Sung Eun Lee

Hankyong National University
Dongbu ARI
CSIRO

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A proteomic study using a PH₃-susceptible (RD2) and -resistant strain (CRD343) of Rhyzopertha dominica was undertaken to validate the relation between change of proteins and PH₃ resistance. Protein expression levels were compared using PD-Quest program after two-dimensional polyacrylamide gel-electrophoresis. Comparing the intensity of proteins, 15 proteins decreased and 6 proteins were newly expressed in CRD343. After MALDI-TOF and LC-MS/MS analyses, the decreased proteins were identified as arginine kinases, dihydrolipoamide dehydrogenase, Hsp60, reverse transcriptase, glyceraldehydes-3-phosphate dehydrogenase, triosephosphate isomerase and hypothetical proteins. Dihydrolipoamide dehydrogenase is involved in the Krebs cycle and glyceraldehydes-3-phosphate dehydrogenase and triosephosphate isomerase are involved in the glycolysis pathway. Among up-regulated proteins, sodium channel, glutamate racemase, enolase and vitellogenin were identified. Taken together, PH₃ affected glycolysis as well as Krebs cycle and the induction of enolase might recover this dysfunction.

Original language	English
Pages (from-to)	121-126
Number of pages	6
Journal	Environmental Toxicology and Pharmacology
Volume	25
Issue number	1
DOIs	https://doi.org/10.1016/j.etap.2007.10.028
State	Published - Jan 2008

Keywords

2D-PAGE
Dihydrolipoamide dehydrogenase
Enolase
MALDI-TOF
Phosphine resistance
Rhyzopertha dominica

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10.1016/j.etap.2007.10.028

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title = "Proteomic evaluation of adults of Rhyzopertha dominica resistant to phosphine",

abstract = "A proteomic study using a PH3-susceptible (RD2) and -resistant strain (CRD343) of Rhyzopertha dominica was undertaken to validate the relation between change of proteins and PH3 resistance. Protein expression levels were compared using PD-Quest program after two-dimensional polyacrylamide gel-electrophoresis. Comparing the intensity of proteins, 15 proteins decreased and 6 proteins were newly expressed in CRD343. After MALDI-TOF and LC-MS/MS analyses, the decreased proteins were identified as arginine kinases, dihydrolipoamide dehydrogenase, Hsp60, reverse transcriptase, glyceraldehydes-3-phosphate dehydrogenase, triosephosphate isomerase and hypothetical proteins. Dihydrolipoamide dehydrogenase is involved in the Krebs cycle and glyceraldehydes-3-phosphate dehydrogenase and triosephosphate isomerase are involved in the glycolysis pathway. Among up-regulated proteins, sodium channel, glutamate racemase, enolase and vitellogenin were identified. Taken together, PH3 affected glycolysis as well as Krebs cycle and the induction of enolase might recover this dysfunction.",

keywords = "2D-PAGE, Dihydrolipoamide dehydrogenase, Enolase, MALDI-TOF, Phosphine resistance, Rhyzopertha dominica",

author = "Park, \{Byeoung Soo\} and Lee, \{Byung Ho\} and Kim, \{Tae Wan\} and Ren, \{Yong Lin\} and Lee, \{Sung Eun\}",

year = "2008",

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doi = "10.1016/j.etap.2007.10.028",

language = "English",

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AU - Park, Byeoung Soo

AU - Lee, Byung Ho

AU - Kim, Tae Wan

AU - Ren, Yong Lin

AU - Lee, Sung Eun

PY - 2008/1

Y1 - 2008/1

N2 - A proteomic study using a PH3-susceptible (RD2) and -resistant strain (CRD343) of Rhyzopertha dominica was undertaken to validate the relation between change of proteins and PH3 resistance. Protein expression levels were compared using PD-Quest program after two-dimensional polyacrylamide gel-electrophoresis. Comparing the intensity of proteins, 15 proteins decreased and 6 proteins were newly expressed in CRD343. After MALDI-TOF and LC-MS/MS analyses, the decreased proteins were identified as arginine kinases, dihydrolipoamide dehydrogenase, Hsp60, reverse transcriptase, glyceraldehydes-3-phosphate dehydrogenase, triosephosphate isomerase and hypothetical proteins. Dihydrolipoamide dehydrogenase is involved in the Krebs cycle and glyceraldehydes-3-phosphate dehydrogenase and triosephosphate isomerase are involved in the glycolysis pathway. Among up-regulated proteins, sodium channel, glutamate racemase, enolase and vitellogenin were identified. Taken together, PH3 affected glycolysis as well as Krebs cycle and the induction of enolase might recover this dysfunction.

AB - A proteomic study using a PH3-susceptible (RD2) and -resistant strain (CRD343) of Rhyzopertha dominica was undertaken to validate the relation between change of proteins and PH3 resistance. Protein expression levels were compared using PD-Quest program after two-dimensional polyacrylamide gel-electrophoresis. Comparing the intensity of proteins, 15 proteins decreased and 6 proteins were newly expressed in CRD343. After MALDI-TOF and LC-MS/MS analyses, the decreased proteins were identified as arginine kinases, dihydrolipoamide dehydrogenase, Hsp60, reverse transcriptase, glyceraldehydes-3-phosphate dehydrogenase, triosephosphate isomerase and hypothetical proteins. Dihydrolipoamide dehydrogenase is involved in the Krebs cycle and glyceraldehydes-3-phosphate dehydrogenase and triosephosphate isomerase are involved in the glycolysis pathway. Among up-regulated proteins, sodium channel, glutamate racemase, enolase and vitellogenin were identified. Taken together, PH3 affected glycolysis as well as Krebs cycle and the induction of enolase might recover this dysfunction.

KW - 2D-PAGE

KW - Dihydrolipoamide dehydrogenase

KW - Enolase

KW - MALDI-TOF

KW - Phosphine resistance

KW - Rhyzopertha dominica

UR - https://www.scopus.com/pages/publications/36749074621

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DO - 10.1016/j.etap.2007.10.028

M3 - Article

AN - SCOPUS:36749074621

SN - 1382-6689

VL - 25

SP - 121

EP - 126

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

IS - 1

ER -

Proteomic evaluation of adults of Rhyzopertha dominica resistant to phosphine

Abstract

Keywords

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