Proteomic evaluation on antiplatelet activity of piperlongumine derived from Piper longum

Sung Eun Lee; Byeoung Soo Park; Tae Lin Huh; Eun Woo Lee; Jong Hwa Yum

doi:10.1007/s13273-010-0040-3

Proteomic evaluation on antiplatelet activity of piperlongumine derived from Piper longum

Sung Eun Lee, Byeoung Soo Park, Tae Lin Huh, Eun Woo Lee, Jong Hwa Yum

School of Applied Bioscience

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Piperlongumine (PL) abundantly present in Piper longum fruits strongly inhibited platelet cell aggregation. Proteomic studies using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and LC-MS/MS analysis showed that PL inhibited collagen-induced aggregation in rabbit platelet cells induced by collagen. Addition of PL protected aggregation of rabbit platelet cells from collagen induction and mediated proteomic changes as 33 proteins decreased and 24 proteins increased. Coagulation factor and fibrinogen were down-regulated and they are involved in aggregation of the platelet cells. Antithrombogenic effect of PL was determined using mice with an i.v. injection of a mixture of collagen and epinephrine. Protection rate of PL on thrombogenic effect of the mixtures was up to 47.9% and it was 8.6% higher than that of aspirin. Taken together, these results strongly suggest that PL is considered as a potent medicine for treating unexpected platelet cell aggregation.

Original language	English
Pages (from-to)	295-303
Number of pages	9
Journal	Molecular and Cellular Toxicology
Volume	6
Issue number	3
DOIs	https://doi.org/10.1007/s13273-010-0040-3
State	Published - Sep 2010

Keywords

2D-PAGE
Antithrombogenic activity
LC-MS/MS
Piperlongumine
Proteomics

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10.1007/s13273-010-0040-3

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title = "Proteomic evaluation on antiplatelet activity of piperlongumine derived from Piper longum",

abstract = "Piperlongumine (PL) abundantly present in Piper longum fruits strongly inhibited platelet cell aggregation. Proteomic studies using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and LC-MS/MS analysis showed that PL inhibited collagen-induced aggregation in rabbit platelet cells induced by collagen. Addition of PL protected aggregation of rabbit platelet cells from collagen induction and mediated proteomic changes as 33 proteins decreased and 24 proteins increased. Coagulation factor and fibrinogen were down-regulated and they are involved in aggregation of the platelet cells. Antithrombogenic effect of PL was determined using mice with an i.v. injection of a mixture of collagen and epinephrine. Protection rate of PL on thrombogenic effect of the mixtures was up to 47.9% and it was 8.6% higher than that of aspirin. Taken together, these results strongly suggest that PL is considered as a potent medicine for treating unexpected platelet cell aggregation.",

keywords = "2D-PAGE, Antithrombogenic activity, LC-MS/MS, Piperlongumine, Proteomics",

author = "Lee, {Sung Eun} and Park, {Byeoung Soo} and Huh, {Tae Lin} and Lee, {Eun Woo} and Yum, {Jong Hwa}",

year = "2010",

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doi = "10.1007/s13273-010-0040-3",

language = "English",

volume = "6",

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journal = "Molecular and Cellular Toxicology",

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T1 - Proteomic evaluation on antiplatelet activity of piperlongumine derived from Piper longum

AU - Lee, Sung Eun

AU - Park, Byeoung Soo

AU - Huh, Tae Lin

AU - Lee, Eun Woo

AU - Yum, Jong Hwa

PY - 2010/9

Y1 - 2010/9

N2 - Piperlongumine (PL) abundantly present in Piper longum fruits strongly inhibited platelet cell aggregation. Proteomic studies using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and LC-MS/MS analysis showed that PL inhibited collagen-induced aggregation in rabbit platelet cells induced by collagen. Addition of PL protected aggregation of rabbit platelet cells from collagen induction and mediated proteomic changes as 33 proteins decreased and 24 proteins increased. Coagulation factor and fibrinogen were down-regulated and they are involved in aggregation of the platelet cells. Antithrombogenic effect of PL was determined using mice with an i.v. injection of a mixture of collagen and epinephrine. Protection rate of PL on thrombogenic effect of the mixtures was up to 47.9% and it was 8.6% higher than that of aspirin. Taken together, these results strongly suggest that PL is considered as a potent medicine for treating unexpected platelet cell aggregation.

AB - Piperlongumine (PL) abundantly present in Piper longum fruits strongly inhibited platelet cell aggregation. Proteomic studies using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and LC-MS/MS analysis showed that PL inhibited collagen-induced aggregation in rabbit platelet cells induced by collagen. Addition of PL protected aggregation of rabbit platelet cells from collagen induction and mediated proteomic changes as 33 proteins decreased and 24 proteins increased. Coagulation factor and fibrinogen were down-regulated and they are involved in aggregation of the platelet cells. Antithrombogenic effect of PL was determined using mice with an i.v. injection of a mixture of collagen and epinephrine. Protection rate of PL on thrombogenic effect of the mixtures was up to 47.9% and it was 8.6% higher than that of aspirin. Taken together, these results strongly suggest that PL is considered as a potent medicine for treating unexpected platelet cell aggregation.

KW - 2D-PAGE

KW - Antithrombogenic activity

KW - LC-MS/MS

KW - Piperlongumine

KW - Proteomics

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U2 - 10.1007/s13273-010-0040-3

DO - 10.1007/s13273-010-0040-3

M3 - Article

AN - SCOPUS:79951885111

SN - 1738-642X

VL - 6

SP - 295

EP - 303

JO - Molecular and Cellular Toxicology

JF - Molecular and Cellular Toxicology

IS - 3

ER -

Proteomic evaluation on antiplatelet activity of piperlongumine derived from Piper longum

Abstract

Keywords

UN SDGs

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