Purification, crystallization and preliminary X-ray analysis of the soluble domain of the Na+-pumping cytochrome bo quinol oxidase from Vitreoscilla

Kyung Jin Kim; Youngchang Kim; Kyung Won Park; Dale A. Webster; Andrew J. Howard

doi:10.1107/S0907444902006170

Purification, crystallization and preliminary X-ray analysis of the soluble domain of the Na⁺-pumping cytochrome bo quinol oxidase from Vitreoscilla

Kyung Jin Kim, Youngchang Kim, Kyung Won Park, Dale A. Webster, Andrew J. Howard

Argonne National Laboratory

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The 24 kDa CyoA soluble domain of Vitreoscilla cytochrome bo quinol oxidase, which pumps out Na⁺ during respiration, has been crystallized from a solution of 2 M ammonium sulfate and 5% 2-propanol. The crystal belongs to cubic space group P4₃32, with unit-cell parameters a = b = c = 122.2 Å, α = β = γ = 90° and one subunit in the asymmetric unit. A 99.8% complete data set to 3.3 Å has been collected at the 17-ID beamline of the Advanced Photon Source. The structure was determined by molecular replacement and refinement is in progress.

Original language	English
Pages (from-to)	1329-1331
Number of pages	3
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	58
Issue number	8
DOIs	https://doi.org/10.1107/S0907444902006170
State	Published - 2002

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title = "Purification, crystallization and preliminary X-ray analysis of the soluble domain of the Na+-pumping cytochrome bo quinol oxidase from Vitreoscilla",

abstract = "The 24 kDa CyoA soluble domain of Vitreoscilla cytochrome bo quinol oxidase, which pumps out Na+ during respiration, has been crystallized from a solution of 2 M ammonium sulfate and 5% 2-propanol. The crystal belongs to cubic space group P4332, with unit-cell parameters a = b = c = 122.2 {\AA}, α = β = γ = 90° and one subunit in the asymmetric unit. A 99.8% complete data set to 3.3 {\AA} has been collected at the 17-ID beamline of the Advanced Photon Source. The structure was determined by molecular replacement and refinement is in progress.",

author = "Kim, {Kyung Jin} and Youngchang Kim and Park, {Kyung Won} and Webster, {Dale A.} and Howard, {Andrew J.}",

year = "2002",

doi = "10.1107/S0907444902006170",

language = "English",

volume = "58",

pages = "1329--1331",

journal = "Acta Crystallographica Section D: Biological Crystallography",

issn = "0907-4449",

publisher = "John Wiley & Sons Inc.",

number = "8",

}

Purification, crystallization and preliminary X-ray analysis of the soluble domain of the Na⁺-pumping cytochrome bo quinol oxidase from Vitreoscilla. / Kim, Kyung Jin; Kim, Youngchang; Park, Kyung Won et al.
In: Acta Crystallographica Section D: Biological Crystallography, Vol. 58, No. 8, 2002, p. 1329-1331.

Research output: Contribution to journal › Article › peer-review

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T1 - Purification, crystallization and preliminary X-ray analysis of the soluble domain of the Na+-pumping cytochrome bo quinol oxidase from Vitreoscilla

AU - Kim, Kyung Jin

AU - Kim, Youngchang

AU - Park, Kyung Won

AU - Webster, Dale A.

AU - Howard, Andrew J.

PY - 2002

Y1 - 2002

N2 - The 24 kDa CyoA soluble domain of Vitreoscilla cytochrome bo quinol oxidase, which pumps out Na+ during respiration, has been crystallized from a solution of 2 M ammonium sulfate and 5% 2-propanol. The crystal belongs to cubic space group P4332, with unit-cell parameters a = b = c = 122.2 Å, α = β = γ = 90° and one subunit in the asymmetric unit. A 99.8% complete data set to 3.3 Å has been collected at the 17-ID beamline of the Advanced Photon Source. The structure was determined by molecular replacement and refinement is in progress.

AB - The 24 kDa CyoA soluble domain of Vitreoscilla cytochrome bo quinol oxidase, which pumps out Na+ during respiration, has been crystallized from a solution of 2 M ammonium sulfate and 5% 2-propanol. The crystal belongs to cubic space group P4332, with unit-cell parameters a = b = c = 122.2 Å, α = β = γ = 90° and one subunit in the asymmetric unit. A 99.8% complete data set to 3.3 Å has been collected at the 17-ID beamline of the Advanced Photon Source. The structure was determined by molecular replacement and refinement is in progress.

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JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 8

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Purification, crystallization and preliminary X-ray analysis of the soluble domain of the Na⁺-pumping cytochrome bo quinol oxidase from Vitreoscilla

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