Purification of outer membrane protein C (ompC) from Salmonella Gallinarum for characterization of its immunogenicity

Young Sung Lee; Kyoung Seong Choi; Myeong Chul Kim; Jae Cheol Han; Jin H.O. Park; Devendra H. Shah; Joon Seok Chae

Purification of outer membrane protein C (ompC) from Salmonella Gallinarum for characterization of its immunogenicity

Young Sung Lee, Kyoung Seong Choi, Myeong Chul Kim, Jae Cheol Han, Jin H.O. Park, Devendra H. Shah, Joon Seok Chae

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Fowl typhoid caused by Salmonella Gallinarum is a severe systemic disease of poultry characterized by high mortality, morbidity, reduced egg production and heavy economic losses due to culling of infected flocks. To investigate antigenicity and immunogenicity, outer membrane protein C (ompC) of S. Gallinarum was extracted, purified and characterized using SDS - polyacrylamide gel electrophoresis, western immunoblot analysis and enzyme linked immunosorbent assay. All the strains of S. Gallinarum tested showed presence of 39 kDa molecular weight ompC. Western blots revealed high intensity of reactivity of the ompC to anti-rabbit ompC sera and to sera from chickens infected with S. Gallinarum. The onipC (39 kDa) of S. Gallinarum effectively induces an immune response and this protein may have a potential for development of diagnostics and subunit vaccine.

Original language	English
Pages (from-to)	164-167
Number of pages	4
Journal	Indian Journal of Animal Sciences
Volume	75
Issue number	2
State	Published - Feb 2005

Keywords

Immunogenicity
Omp
Outer membrane protein
Salmonella gallinarum

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Purification of outer membrane protein C (ompC) from Salmonella Gallinarum for characterization of its immunogenicity",

abstract = "Fowl typhoid caused by Salmonella Gallinarum is a severe systemic disease of poultry characterized by high mortality, morbidity, reduced egg production and heavy economic losses due to culling of infected flocks. To investigate antigenicity and immunogenicity, outer membrane protein C (ompC) of S. Gallinarum was extracted, purified and characterized using SDS - polyacrylamide gel electrophoresis, western immunoblot analysis and enzyme linked immunosorbent assay. All the strains of S. Gallinarum tested showed presence of 39 kDa molecular weight ompC. Western blots revealed high intensity of reactivity of the ompC to anti-rabbit ompC sera and to sera from chickens infected with S. Gallinarum. The onipC (39 kDa) of S. Gallinarum effectively induces an immune response and this protein may have a potential for development of diagnostics and subunit vaccine.",

keywords = "Immunogenicity, Omp, Outer membrane protein, Salmonella gallinarum",

author = "Lee, {Young Sung} and Choi, {Kyoung Seong} and Kim, {Myeong Chul} and Han, {Jae Cheol} and Park, {Jin H.O.} and Shah, {Devendra H.} and Chae, {Joon Seok}",

year = "2005",

month = feb,

language = "English",

volume = "75",

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journal = "Indian Journal of Animal Sciences",

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publisher = "Indian Council of Agricultural Research",

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TY - JOUR

T1 - Purification of outer membrane protein C (ompC) from Salmonella Gallinarum for characterization of its immunogenicity

AU - Lee, Young Sung

AU - Choi, Kyoung Seong

AU - Kim, Myeong Chul

AU - Han, Jae Cheol

AU - Park, Jin H.O.

AU - Shah, Devendra H.

AU - Chae, Joon Seok

PY - 2005/2

Y1 - 2005/2

N2 - Fowl typhoid caused by Salmonella Gallinarum is a severe systemic disease of poultry characterized by high mortality, morbidity, reduced egg production and heavy economic losses due to culling of infected flocks. To investigate antigenicity and immunogenicity, outer membrane protein C (ompC) of S. Gallinarum was extracted, purified and characterized using SDS - polyacrylamide gel electrophoresis, western immunoblot analysis and enzyme linked immunosorbent assay. All the strains of S. Gallinarum tested showed presence of 39 kDa molecular weight ompC. Western blots revealed high intensity of reactivity of the ompC to anti-rabbit ompC sera and to sera from chickens infected with S. Gallinarum. The onipC (39 kDa) of S. Gallinarum effectively induces an immune response and this protein may have a potential for development of diagnostics and subunit vaccine.

AB - Fowl typhoid caused by Salmonella Gallinarum is a severe systemic disease of poultry characterized by high mortality, morbidity, reduced egg production and heavy economic losses due to culling of infected flocks. To investigate antigenicity and immunogenicity, outer membrane protein C (ompC) of S. Gallinarum was extracted, purified and characterized using SDS - polyacrylamide gel electrophoresis, western immunoblot analysis and enzyme linked immunosorbent assay. All the strains of S. Gallinarum tested showed presence of 39 kDa molecular weight ompC. Western blots revealed high intensity of reactivity of the ompC to anti-rabbit ompC sera and to sera from chickens infected with S. Gallinarum. The onipC (39 kDa) of S. Gallinarum effectively induces an immune response and this protein may have a potential for development of diagnostics and subunit vaccine.

KW - Immunogenicity

KW - Omp

KW - Outer membrane protein

KW - Salmonella gallinarum

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M3 - Article

AN - SCOPUS:18844440408

SN - 0367-8318

VL - 75

SP - 164

EP - 167

JO - Indian Journal of Animal Sciences

JF - Indian Journal of Animal Sciences

IS - 2

ER -

Purification of outer membrane protein C (ompC) from Salmonella Gallinarum for characterization of its immunogenicity

Abstract

Keywords

UN SDGs

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