QTL mapping and DNA marker selection associated with root growth of rice seedlings

Kyung Min Kim, Chang Hoon Kim, Yong Sham Kwon, Jae Keun Sohn

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of this study was to map the quantitative trait loci (QTL) associated with root growth of rice using the 164 recombinant inbred (RI) lines derived from a cross between 'Milyang 23/Gihobyeo' and to validate the effectiveness for improvement of seedling vigor using marker-assisted selection (MAS). Frequency of root length of RI population grown for 10 days at 18°C and room temperature in greenhouse showed nearly normal distributions with a transgressive segregation. Two putative QTLs related to root growth were located on chromosomes 1 and 12. The QTL located on chromosome 1 had the same map position in different screening methods. This QTL accounted for 32.5% of total phenotypic variation and acted additively on the root growth in the green house. The relationship between the DNA marker (RM5) linked with this QTL and root growth of 19 rice varieties was confirmed to high significance. MAS of the major QTL for root growth can improve selection efficiency and may facilitate stacking of genes associated with root growth ability from different sources.

Original languageEnglish
Pages (from-to)317-322
Number of pages6
JournalKorean Journal of Genetics
Volume25
Issue number4
StatePublished - Dec 2003

Keywords

  • Marker-assisted Selection
  • QTL Mapping
  • Rice
  • Seedling Vigor

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