Abstract
This study demonstrates a non-destructive histomorphometric analysis technique that uses virtual tissue slices obtained with synchrotron radiation X-ray microtomography (SR-μCT), and compared the results to those obtained using conventional hematoxylin-eosin (H&E) photomicrography of regenerated bone in rat calvarial defects grafted with deproteinized bovine bone substitute (Bio-Oss®). Calvarial defects (diameter, 4 mm) were created in 8 adult male Spraque-Dawley rats and filled with Bio-Oss®. The percentage of newly formed bone (NB%) was evaluated histomorphometrically using SR-μCT images and photomicrographs (H&E) 3 weeks postoperatively. Osteoconductive new bone formation was observed in the calvarial central defect. Histomorphometric analysis revealed no NB% differences between the SR-μCT and photomicrograph (H&E) groups at 3 weeks (p=0.959). The mean new bone area fractions were 17.62% and 17.95% in the analysis using SR-μCT virtual slices and conventional H&E-stained slices, respectively. Therefore, Bio-Oss® bone regeneration can be quantified non-destructively using SR-μCT at an accuracy comparable to that obtained using conventional photomicrography.
Original language | English |
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Pages (from-to) | 379-385 |
Number of pages | 7 |
Journal | Tissue Engineering and Regenerative Medicine |
Volume | 12 |
Issue number | 5 |
DOIs | |
State | Published - 1 Oct 2015 |
Keywords
- Bone substitute
- Histomorphometry
- Photomicrograph (H&E)
- Radiation X-ray microtomography