Quantification of bone regeneration by virtual slices using non-destructive synchrotron X-ray microtomography

Yong Gun Kim; Chung Wung Bark

doi:10.1007/s13770-015-0003-9

Quantification of bone regeneration by virtual slices using non-destructive synchrotron X-ray microtomography

Yong Gun Kim, Chung Wung Bark

Department of Dentistry

Gachon University

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

This study demonstrates a non-destructive histomorphometric analysis technique that uses virtual tissue slices obtained with synchrotron radiation X-ray microtomography (SR-μCT), and compared the results to those obtained using conventional hematoxylin-eosin (H&E) photomicrography of regenerated bone in rat calvarial defects grafted with deproteinized bovine bone substitute (Bio-Oss^®). Calvarial defects (diameter, 4 mm) were created in 8 adult male Spraque-Dawley rats and filled with Bio-Oss^®. The percentage of newly formed bone (NB%) was evaluated histomorphometrically using SR-μCT images and photomicrographs (H&E) 3 weeks postoperatively. Osteoconductive new bone formation was observed in the calvarial central defect. Histomorphometric analysis revealed no NB% differences between the SR-μCT and photomicrograph (H&E) groups at 3 weeks (p=0.959). The mean new bone area fractions were 17.62% and 17.95% in the analysis using SR-μCT virtual slices and conventional H&E-stained slices, respectively. Therefore, Bio-Oss^® bone regeneration can be quantified non-destructively using SR-μCT at an accuracy comparable to that obtained using conventional photomicrography.

Original language	English
Pages (from-to)	379-385
Number of pages	7
Journal	Tissue Engineering and Regenerative Medicine
Volume	12
Issue number	5
DOIs	https://doi.org/10.1007/s13770-015-0003-9
State	Published - 1 Oct 2015

Keywords

Bone substitute
Histomorphometry
Photomicrograph (H&E)
Radiation X-ray microtomography

Access to Document

10.1007/s13770-015-0003-9

Cite this

@article{6650644a85fe4b2c97be5bb73bfac642,

title = "Quantification of bone regeneration by virtual slices using non-destructive synchrotron X-ray microtomography",

abstract = "This study demonstrates a non-destructive histomorphometric analysis technique that uses virtual tissue slices obtained with synchrotron radiation X-ray microtomography (SR-μCT), and compared the results to those obtained using conventional hematoxylin-eosin (H&E) photomicrography of regenerated bone in rat calvarial defects grafted with deproteinized bovine bone substitute (Bio-Oss{\textregistered}). Calvarial defects (diameter, 4 mm) were created in 8 adult male Spraque-Dawley rats and filled with Bio-Oss{\textregistered}. The percentage of newly formed bone (NB%) was evaluated histomorphometrically using SR-μCT images and photomicrographs (H&E) 3 weeks postoperatively. Osteoconductive new bone formation was observed in the calvarial central defect. Histomorphometric analysis revealed no NB% differences between the SR-μCT and photomicrograph (H&E) groups at 3 weeks (p=0.959). The mean new bone area fractions were 17.62% and 17.95% in the analysis using SR-μCT virtual slices and conventional H&E-stained slices, respectively. Therefore, Bio-Oss{\textregistered} bone regeneration can be quantified non-destructively using SR-μCT at an accuracy comparable to that obtained using conventional photomicrography.",

keywords = "Bone substitute, Histomorphometry, Photomicrograph (H&E), Radiation X-ray microtomography",

author = "Kim, {Yong Gun} and Bark, {Chung Wung}",

note = "Publisher Copyright: {\textcopyright} 2015, The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht.",

year = "2015",

month = oct,

day = "1",

doi = "10.1007/s13770-015-0003-9",

language = "English",

volume = "12",

pages = "379--385",

journal = "Tissue Engineering and Regenerative Medicine",

issn = "1738-2696",

publisher = "Springer Science + Business Media",

number = "5",

}

TY - JOUR

T1 - Quantification of bone regeneration by virtual slices using non-destructive synchrotron X-ray microtomography

AU - Kim, Yong Gun

AU - Bark, Chung Wung

PY - 2015/10/1

Y1 - 2015/10/1

N2 - This study demonstrates a non-destructive histomorphometric analysis technique that uses virtual tissue slices obtained with synchrotron radiation X-ray microtomography (SR-μCT), and compared the results to those obtained using conventional hematoxylin-eosin (H&E) photomicrography of regenerated bone in rat calvarial defects grafted with deproteinized bovine bone substitute (Bio-Oss®). Calvarial defects (diameter, 4 mm) were created in 8 adult male Spraque-Dawley rats and filled with Bio-Oss®. The percentage of newly formed bone (NB%) was evaluated histomorphometrically using SR-μCT images and photomicrographs (H&E) 3 weeks postoperatively. Osteoconductive new bone formation was observed in the calvarial central defect. Histomorphometric analysis revealed no NB% differences between the SR-μCT and photomicrograph (H&E) groups at 3 weeks (p=0.959). The mean new bone area fractions were 17.62% and 17.95% in the analysis using SR-μCT virtual slices and conventional H&E-stained slices, respectively. Therefore, Bio-Oss® bone regeneration can be quantified non-destructively using SR-μCT at an accuracy comparable to that obtained using conventional photomicrography.

AB - This study demonstrates a non-destructive histomorphometric analysis technique that uses virtual tissue slices obtained with synchrotron radiation X-ray microtomography (SR-μCT), and compared the results to those obtained using conventional hematoxylin-eosin (H&E) photomicrography of regenerated bone in rat calvarial defects grafted with deproteinized bovine bone substitute (Bio-Oss®). Calvarial defects (diameter, 4 mm) were created in 8 adult male Spraque-Dawley rats and filled with Bio-Oss®. The percentage of newly formed bone (NB%) was evaluated histomorphometrically using SR-μCT images and photomicrographs (H&E) 3 weeks postoperatively. Osteoconductive new bone formation was observed in the calvarial central defect. Histomorphometric analysis revealed no NB% differences between the SR-μCT and photomicrograph (H&E) groups at 3 weeks (p=0.959). The mean new bone area fractions were 17.62% and 17.95% in the analysis using SR-μCT virtual slices and conventional H&E-stained slices, respectively. Therefore, Bio-Oss® bone regeneration can be quantified non-destructively using SR-μCT at an accuracy comparable to that obtained using conventional photomicrography.

KW - Bone substitute

KW - Histomorphometry

KW - Photomicrograph (H&E)

KW - Radiation X-ray microtomography

UR - http://www.scopus.com/inward/record.url?scp=84944744659&partnerID=8YFLogxK

U2 - 10.1007/s13770-015-0003-9

DO - 10.1007/s13770-015-0003-9

M3 - Article

AN - SCOPUS:84944744659

SN - 1738-2696

VL - 12

SP - 379

EP - 385

JO - Tissue Engineering and Regenerative Medicine

JF - Tissue Engineering and Regenerative Medicine

IS - 5

ER -

Quantification of bone regeneration by virtual slices using non-destructive synchrotron X-ray microtomography

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this