Quantification of enterocytozoon hepatopenaei (Ehp) in penaeid shrimps from southeast asia and latin america using taqman probe-based quantitative PCR

Patharapol Piamsomboon; Seong Kyoon Choi; Bambang Hanggono; Yani Lestari Nuraini; Fatma Wati; Kathy F.J. Tang; Song Park; Dongmi Kwak; Man Hee Rhee; Jee Eun Han; Ji Hyung Kim

doi:10.3390/pathogens8040233

Quantification of enterocytozoon hepatopenaei (Ehp) in penaeid shrimps from southeast asia and latin america using taqman probe-based quantitative PCR

Patharapol Piamsomboon
, Seong Kyoon Choi
, Bambang Hanggono
, Yani Lestari Nuraini
, Fatma Wati
, Kathy F.J. Tang
, Song Park
, Dongmi Kwak
, Man Hee Rhee
, Jee Eun Han
, Ji Hyung Kim

Department of Veterinary Medicine

Chulalongkorn University
Daegu Gyeongbuk Institute of Science and Technology
Brackishwater Aquaculture Development Center Situbondo
Chinese Academy of Fishery Sciences
Korea Research Institute of Bioscience and Biotechnology

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (10⁶–10⁸ copies mg⁻¹), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (10¹–10³ copies mg⁻¹ or mL⁻¹ of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.

Original language	English
Article number	233
Journal	Pathogens
Volume	8
Issue number	4
DOIs	https://doi.org/10.3390/pathogens8040233
State	Published - Dec 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Enterocytozoon hepatopenaei
Microsporidian
QPCR
Shrimp parasite

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10.3390/pathogens8040233

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Piamsomboon, P., Choi, S. K., Hanggono, B., Nuraini, Y. L., Wati, F., Tang, K. F. J., Park, S., Kwak, D., Rhee, M. H., Han, J. E., & Kim, J. H. (2019). Quantification of enterocytozoon hepatopenaei (Ehp) in penaeid shrimps from southeast asia and latin america using taqman probe-based quantitative PCR. Pathogens, 8(4), Article 233. https://doi.org/10.3390/pathogens8040233

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title = "Quantification of enterocytozoon hepatopenaei (Ehp) in penaeid shrimps from southeast asia and latin america using taqman probe-based quantitative PCR",

abstract = "We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106–108 copies mg−1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101–103 copies mg−1 or mL−1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.",

keywords = "Enterocytozoon hepatopenaei, Microsporidian, QPCR, Shrimp parasite",

author = "Patharapol Piamsomboon and Choi, \{Seong Kyoon\} and Bambang Hanggono and Nuraini, \{Yani Lestari\} and Fatma Wati and Tang, \{Kathy F.J.\} and Song Park and Dongmi Kwak and Rhee, \{Man Hee\} and Han, \{Jee Eun\} and Kim, \{Ji Hyung\}",

note = "Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2019",

month = dec,

doi = "10.3390/pathogens8040233",

language = "English",

volume = "8",

journal = "Pathogens",

issn = "2076-0817",

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T1 - Quantification of enterocytozoon hepatopenaei (Ehp) in penaeid shrimps from southeast asia and latin america using taqman probe-based quantitative PCR

AU - Piamsomboon, Patharapol

AU - Choi, Seong Kyoon

AU - Hanggono, Bambang

AU - Nuraini, Yani Lestari

AU - Wati, Fatma

AU - Tang, Kathy F.J.

AU - Park, Song

AU - Kwak, Dongmi

AU - Rhee, Man Hee

AU - Han, Jee Eun

AU - Kim, Ji Hyung

PY - 2019/12

Y1 - 2019/12

N2 - We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106–108 copies mg−1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101–103 copies mg−1 or mL−1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.

AB - We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (106–108 copies mg−1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (101–103 copies mg−1 or mL−1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.

KW - Enterocytozoon hepatopenaei

KW - Microsporidian

KW - QPCR

KW - Shrimp parasite

UR - https://www.scopus.com/pages/publications/85075060781

U2 - 10.3390/pathogens8040233

DO - 10.3390/pathogens8040233

M3 - Article

AN - SCOPUS:85075060781

SN - 2076-0817

VL - 8

JO - Pathogens

JF - Pathogens

IS - 4

M1 - 233

ER -

Quantification of enterocytozoon hepatopenaei (Ehp) in penaeid shrimps from southeast asia and latin america using taqman probe-based quantitative PCR

Abstract

UN SDGs

Keywords

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