Quantitative ultrastructural analysis of fibers expressing parvalbumin, calretinin, calbindin D-28k, stage specific embryonic antigen-4, and phosphorylated neurofilament 200 in the peripheral sensory root of the rat trigeminal ganglion

Jin Young Bae, Cheol Ju Mun, Yun Sook Kim, Dong Kuk Ahn, Yong Chul Bae

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8 Scopus citations

Abstract

Parvalbumin (PV), calretinin (CR), calbindin D-28k (CB), stage specific embryonic antigen-4 (SSEA4), and phosphorylated neurofilament 200 (pNF200) have been commonly used as markers for primary afferent neurons with large myelinated (A) fibers but detailed information on the expression of these markers in specific primary afferent fiber types is still lacking. We here examined the fibers that express PV, CR, CB, SSEA4, and pNF200 in the trigeminal ganglion and its peripheral sensory root by light- and electron-microscopic immunohistochemistry and quantitative analysis. We found that all CR-immunopositive (+), CB+, and SSEA4+ fibers and virtually all (98.8%) PV+ fibers were myelinated, most CR+ fibers were large myelinated, whereas most CB+ and SSEA4+ fibers were small myelinated. One half of the PV+ fibers were small myelinated and the other half were large myelinated. Of all pNF200+ fibers, about a third each were small myelinated, large myelinated, and unmyelinated. These findings suggest that PV, CR, CB, and SSEA4 can be used as specific markers for primary afferent neurons with myelinated fibers, but that pNF200 is not suitable as a specific marker for primary afferent neurons with myelinated fibers, and also raise the possibility that PV, CR, CB, and SSEA4 may be expressed in both mechanoreceptive and nociceptive neurons.

Original languageEnglish
Pages (from-to)2204-2214
Number of pages11
JournalJournal of Comparative Neurology
Volume526
Issue number14
DOIs
StatePublished - 1 Oct 2018

Keywords

  • fiber type
  • neuronal marker
  • RRID:AB_10000320
  • RRID:AB_10000343
  • RRID:AB_476894
  • RRID:AB_528477
  • RRID:AB_95186
  • RRID:nif-0000-30467
  • sensory root
  • trigeminal ganglion
  • ultrastructure

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