Quencher-free linear beacon systems containing 2-ethynylfluorenone-labeled 2′-deoxyuridine units

Jin Ho Ryu; Ju Young Heo; Eun Kyoung Bang; Gil Tae Hwang; Byeang Hyean Kim

doi:10.1016/j.tet.2011.10.092

Quencher-free linear beacon systems containing 2-ethynylfluorenone-labeled 2′-deoxyuridine units

Jin Ho Ryu, Ju Young Heo, Eun Kyoung Bang, Gil Tae Hwang, Byeang Hyean Kim

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

In this study we synthesized the 2-ethynylfluorenone-containing deoxyuridine derivative U^FO and incorporated it at the central positions of oligodeoxynucleotides (ODNs) in an attempt to develop a new type of quencher-free linear beacon probe and to understand the effect of functionalization of the fluorene scaffold on the photophysical properties of the fluorescent ODNs. Herein, we describe our photophysical studies of U ^FO DNA probes and their application to single-nucleotide polymorphism typing.

Original language	English
Pages (from-to)	72-78
Number of pages	7
Journal	Tetrahedron
Volume	68
Issue number	1
DOIs	https://doi.org/10.1016/j.tet.2011.10.092
State	Published - 7 Jan 2012

Keywords

DNA probe
Fluorenone
Fluorescence
Oligonucleotides
Single-nucleotide polymorphism

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10.1016/j.tet.2011.10.092

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title = "Quencher-free linear beacon systems containing 2-ethynylfluorenone-labeled 2′-deoxyuridine units",

abstract = "In this study we synthesized the 2-ethynylfluorenone-containing deoxyuridine derivative UFO and incorporated it at the central positions of oligodeoxynucleotides (ODNs) in an attempt to develop a new type of quencher-free linear beacon probe and to understand the effect of functionalization of the fluorene scaffold on the photophysical properties of the fluorescent ODNs. Herein, we describe our photophysical studies of U FO DNA probes and their application to single-nucleotide polymorphism typing.",

keywords = "DNA probe, Fluorenone, Fluorescence, Oligonucleotides, Single-nucleotide polymorphism",

author = "Ryu, {Jin Ho} and Heo, {Ju Young} and Bang, {Eun Kyoung} and Hwang, {Gil Tae} and Kim, {Byeang Hyean}",

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T1 - Quencher-free linear beacon systems containing 2-ethynylfluorenone-labeled 2′-deoxyuridine units

AU - Ryu, Jin Ho

AU - Heo, Ju Young

AU - Bang, Eun Kyoung

AU - Hwang, Gil Tae

AU - Kim, Byeang Hyean

PY - 2012/1/7

Y1 - 2012/1/7

N2 - In this study we synthesized the 2-ethynylfluorenone-containing deoxyuridine derivative UFO and incorporated it at the central positions of oligodeoxynucleotides (ODNs) in an attempt to develop a new type of quencher-free linear beacon probe and to understand the effect of functionalization of the fluorene scaffold on the photophysical properties of the fluorescent ODNs. Herein, we describe our photophysical studies of U FO DNA probes and their application to single-nucleotide polymorphism typing.

AB - In this study we synthesized the 2-ethynylfluorenone-containing deoxyuridine derivative UFO and incorporated it at the central positions of oligodeoxynucleotides (ODNs) in an attempt to develop a new type of quencher-free linear beacon probe and to understand the effect of functionalization of the fluorene scaffold on the photophysical properties of the fluorescent ODNs. Herein, we describe our photophysical studies of U FO DNA probes and their application to single-nucleotide polymorphism typing.

KW - DNA probe

KW - Fluorenone

KW - Fluorescence

KW - Oligonucleotides

KW - Single-nucleotide polymorphism

UR - http://www.scopus.com/inward/record.url?scp=82255193946&partnerID=8YFLogxK

U2 - 10.1016/j.tet.2011.10.092

DO - 10.1016/j.tet.2011.10.092

M3 - Article

AN - SCOPUS:82255193946

SN - 0040-4020

VL - 68

SP - 72

EP - 78

JO - Tetrahedron

JF - Tetrahedron

IS - 1

ER -

Quencher-free linear beacon systems containing 2-ethynylfluorenone-labeled 2′-deoxyuridine units

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Keywords

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