Radioprotective effect of hesperetin against γ-irradiation-induced DNA damage and immune dysfunction in murine splenocytes

Jung Ae Kang; Seon Hye Yoon; Jong Kook Rho; Beom su Jang; Dae Seong Choi; Dong Eun Lee; Eui Baek Byun; Jongho Jeon; Sang Hyun Park

doi:10.1007/s10068-016-0114-7

Radioprotective effect of hesperetin against γ-irradiation-induced DNA damage and immune dysfunction in murine splenocytes

Jung Ae Kang, Seon Hye Yoon, Jong Kook Rho, Beom su Jang, Dae Seong Choi, Dong Eun Lee, Eui Baek Byun, Jongho Jeon, Sang Hyun Park

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

This study was conducted to evaluate the preventive effect of hesperetin against radiation-induced DNA damage and immune dysfunction in murine splenocytes. Isolated splenocytes from BALB/c mice were treated with hesperetin (20, 100, and 500 µM), and then irradiated at a dose of 2 and 4 Gy of γ-irradiation. Exposure to ?-radiation resulted in DNA damage and a reduction of cell viability as well as an elevation of the levels of proinflammatory cytokines, intracellular ROS (reactive oxygen species), and NO (nitric oxide). Hesperetin significantly enhanced the cell viability of the splenocytes compared with the irradiated group. In addition, hesperetin was found to be highly effective in preventing DNA damage as identified by comet and DNA ladder assays. Hesperetin also effectively inhibited proinflammatory cytokines, intracellular ROS, and NO in irradiated splenocytes. In conclusion, hesperetin was shown to be radioprotective against irradiation-induced DNA damage and immune dysfunction in murine splenocytes.

Original language	English
Pages (from-to)	163-168
Number of pages	6
Journal	Food Science and Biotechnology
Volume	25
DOIs	https://doi.org/10.1007/s10068-016-0114-7
State	Published - 1 Mar 2016

Keywords

DNA damage
Hesperetin
immune dysfunction
murine splenocytes
γ-irradiation

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title = "Radioprotective effect of hesperetin against γ-irradiation-induced DNA damage and immune dysfunction in murine splenocytes",

abstract = "This study was conducted to evaluate the preventive effect of hesperetin against radiation-induced DNA damage and immune dysfunction in murine splenocytes. Isolated splenocytes from BALB/c mice were treated with hesperetin (20, 100, and 500 µM), and then irradiated at a dose of 2 and 4 Gy of γ-irradiation. Exposure to ?-radiation resulted in DNA damage and a reduction of cell viability as well as an elevation of the levels of proinflammatory cytokines, intracellular ROS (reactive oxygen species), and NO (nitric oxide). Hesperetin significantly enhanced the cell viability of the splenocytes compared with the irradiated group. In addition, hesperetin was found to be highly effective in preventing DNA damage as identified by comet and DNA ladder assays. Hesperetin also effectively inhibited proinflammatory cytokines, intracellular ROS, and NO in irradiated splenocytes. In conclusion, hesperetin was shown to be radioprotective against irradiation-induced DNA damage and immune dysfunction in murine splenocytes.",

keywords = "DNA damage, Hesperetin, immune dysfunction, murine splenocytes, γ-irradiation",

author = "Kang, {Jung Ae} and Yoon, {Seon Hye} and Rho, {Jong Kook} and Jang, {Beom su} and Choi, {Dae Seong} and Lee, {Dong Eun} and Byun, {Eui Baek} and Jongho Jeon and Park, {Sang Hyun}",

note = "Publisher Copyright: {\textcopyright} 2016, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht.",

year = "2016",

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doi = "10.1007/s10068-016-0114-7",

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T1 - Radioprotective effect of hesperetin against γ-irradiation-induced DNA damage and immune dysfunction in murine splenocytes

AU - Kang, Jung Ae

AU - Yoon, Seon Hye

AU - Rho, Jong Kook

AU - Jang, Beom su

AU - Choi, Dae Seong

AU - Lee, Dong Eun

AU - Byun, Eui Baek

AU - Jeon, Jongho

AU - Park, Sang Hyun

PY - 2016/3/1

Y1 - 2016/3/1

N2 - This study was conducted to evaluate the preventive effect of hesperetin against radiation-induced DNA damage and immune dysfunction in murine splenocytes. Isolated splenocytes from BALB/c mice were treated with hesperetin (20, 100, and 500 µM), and then irradiated at a dose of 2 and 4 Gy of γ-irradiation. Exposure to ?-radiation resulted in DNA damage and a reduction of cell viability as well as an elevation of the levels of proinflammatory cytokines, intracellular ROS (reactive oxygen species), and NO (nitric oxide). Hesperetin significantly enhanced the cell viability of the splenocytes compared with the irradiated group. In addition, hesperetin was found to be highly effective in preventing DNA damage as identified by comet and DNA ladder assays. Hesperetin also effectively inhibited proinflammatory cytokines, intracellular ROS, and NO in irradiated splenocytes. In conclusion, hesperetin was shown to be radioprotective against irradiation-induced DNA damage and immune dysfunction in murine splenocytes.

AB - This study was conducted to evaluate the preventive effect of hesperetin against radiation-induced DNA damage and immune dysfunction in murine splenocytes. Isolated splenocytes from BALB/c mice were treated with hesperetin (20, 100, and 500 µM), and then irradiated at a dose of 2 and 4 Gy of γ-irradiation. Exposure to ?-radiation resulted in DNA damage and a reduction of cell viability as well as an elevation of the levels of proinflammatory cytokines, intracellular ROS (reactive oxygen species), and NO (nitric oxide). Hesperetin significantly enhanced the cell viability of the splenocytes compared with the irradiated group. In addition, hesperetin was found to be highly effective in preventing DNA damage as identified by comet and DNA ladder assays. Hesperetin also effectively inhibited proinflammatory cytokines, intracellular ROS, and NO in irradiated splenocytes. In conclusion, hesperetin was shown to be radioprotective against irradiation-induced DNA damage and immune dysfunction in murine splenocytes.

KW - DNA damage

KW - Hesperetin

KW - immune dysfunction

KW - murine splenocytes

KW - γ-irradiation

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DO - 10.1007/s10068-016-0114-7

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SN - 1226-7708

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EP - 168

JO - Food Science and Biotechnology

JF - Food Science and Biotechnology

ER -

Radioprotective effect of hesperetin against γ-irradiation-induced DNA damage and immune dysfunction in murine splenocytes

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