Radiosynthesis and preliminary biological evaluation of 99mTc-labeled 2-methyl-2-pentylmalonic acid as an apoptosis imaging agent

Sajid Mushtaq; Jongho Jeon; Jung Ae Kang; You Ree Nam; Beom Su Jang; Sang Hyun Park

doi:10.1007/s10967-017-5275-1

Radiosynthesis and preliminary biological evaluation of ^99mTc-labeled 2-methyl-2-pentylmalonic acid as an apoptosis imaging agent

Sajid Mushtaq, Jongho Jeon, Jung Ae Kang, You Ree Nam, Beom Su Jang, Sang Hyun Park

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Apoptosis is one of the fundamental phenomena behind successful radiation and chemotherapy treatments. Non-invasive imaging of apoptosis can offer an early diagnosis of disease and the true efficiency of an ongoing treatment procedure. The present study describes an attempt to develop ^99mTc-labeled 2-methyl-2-pentylmalonic acid ([^99mTc] 8) as a new SPECT based apoptosis imaging agent. An optimized chemical and radiosynthesis procedure provided desired product [^99mTc] 8 with high radiochemical yield (84%, n = 3) and radiochemical purity (>99%) as determined by radio HPLC. Biodistribution data indicated that the radiotracer has a rapid clearance from blood and other background tissues. High testes accumulation confirmed the ability of the radiotracer to detect testicular apoptosis in mice.

Original language	English
Pages (from-to)	207-215
Number of pages	9
Journal	Journal of Radioanalytical and Nuclear Chemistry
Volume	313
Issue number	1
DOIs	https://doi.org/10.1007/s10967-017-5275-1
State	Published - 1 Jul 2017

Keywords

Tc-labeling
Apoptosis
Biodistribution
SPECT imaging

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s10967-017-5275-1

Cite this

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title = "Radiosynthesis and preliminary biological evaluation of 99mTc-labeled 2-methyl-2-pentylmalonic acid as an apoptosis imaging agent",

abstract = "Apoptosis is one of the fundamental phenomena behind successful radiation and chemotherapy treatments. Non-invasive imaging of apoptosis can offer an early diagnosis of disease and the true efficiency of an ongoing treatment procedure. The present study describes an attempt to develop 99mTc-labeled 2-methyl-2-pentylmalonic acid ([99mTc] 8) as a new SPECT based apoptosis imaging agent. An optimized chemical and radiosynthesis procedure provided desired product [99mTc] 8 with high radiochemical yield (84\%, n = 3) and radiochemical purity (>99\%) as determined by radio HPLC. Biodistribution data indicated that the radiotracer has a rapid clearance from blood and other background tissues. High testes accumulation confirmed the ability of the radiotracer to detect testicular apoptosis in mice.",

keywords = "Tc-labeling, Apoptosis, Biodistribution, SPECT imaging",

author = "Sajid Mushtaq and Jongho Jeon and Kang, \{Jung Ae\} and Nam, \{You Ree\} and Jang, \{Beom Su\} and Park, \{Sang Hyun\}",

note = "Publisher Copyright: {\textcopyright} 2017, Akad{\'e}miai Kiad{\'o}, Budapest, Hungary.",

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AU - Mushtaq, Sajid

AU - Jeon, Jongho

AU - Kang, Jung Ae

AU - Nam, You Ree

AU - Jang, Beom Su

AU - Park, Sang Hyun

PY - 2017/7/1

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AB - Apoptosis is one of the fundamental phenomena behind successful radiation and chemotherapy treatments. Non-invasive imaging of apoptosis can offer an early diagnosis of disease and the true efficiency of an ongoing treatment procedure. The present study describes an attempt to develop 99mTc-labeled 2-methyl-2-pentylmalonic acid ([99mTc] 8) as a new SPECT based apoptosis imaging agent. An optimized chemical and radiosynthesis procedure provided desired product [99mTc] 8 with high radiochemical yield (84%, n = 3) and radiochemical purity (>99%) as determined by radio HPLC. Biodistribution data indicated that the radiotracer has a rapid clearance from blood and other background tissues. High testes accumulation confirmed the ability of the radiotracer to detect testicular apoptosis in mice.

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KW - Biodistribution

KW - SPECT imaging

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