Rapid and simple procedure for homogenizing leaf tissues suitable for mini-midi-scale DNA extraction in rice

Gihwan Yi; Jun Ho Choi; Jong Hee Lee; Unggi Jeong; Min Hee Nam; Doh Won Yun; Moo Young Eun

doi:10.1081/PB-200065652

Rapid and simple procedure for homogenizing leaf tissues suitable for mini-midi-scale DNA extraction in rice

Gihwan Yi
, Jun Ho Choi
, Jong Hee Lee
, Unggi Jeong
, Min Hee Nam
, Doh Won Yun
, Moo Young Eun

Department of Farm Management

Yeongnam Agricultural Research Institute
Rural Development Administration

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a rapid and simple procedure for homogenizing leaf samples suitable for mini/midi-scale DNA preparation in rice. The methods used tungsten carbide beads and general vortexer for homogenizing leaf samples. In general, two samples can be ground completely within 11.3 ± 1.5 sec at one time. Up to 20 samples can be ground at a time using a vortexer attachment. The yields of the DNA ranged from 2.2 to 7.6 μg from 25-150 mg of young fresh leaf tissue. The quality and quantity of DNA was compatible for most of PCR work and RFLP analysis.

Original language	English
Pages (from-to)	257-262
Number of pages	6
Journal	Preparative Biochemistry and Biotechnology
Volume	35
Issue number	3
DOIs	https://doi.org/10.1081/PB-200065652
State	Published - 2005

Keywords

DNA
Mini/midi-preparation
Tungsten beads
Vortexer

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10.1081/PB-200065652

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title = "Rapid and simple procedure for homogenizing leaf tissues suitable for mini-midi-scale DNA extraction in rice",

abstract = "We describe a rapid and simple procedure for homogenizing leaf samples suitable for mini/midi-scale DNA preparation in rice. The methods used tungsten carbide beads and general vortexer for homogenizing leaf samples. In general, two samples can be ground completely within 11.3 ± 1.5 sec at one time. Up to 20 samples can be ground at a time using a vortexer attachment. The yields of the DNA ranged from 2.2 to 7.6 μg from 25-150 mg of young fresh leaf tissue. The quality and quantity of DNA was compatible for most of PCR work and RFLP analysis.",

keywords = "DNA, Mini/midi-preparation, Tungsten beads, Vortexer",

author = "Gihwan Yi and Choi, \{Jun Ho\} and Lee, \{Jong Hee\} and Unggi Jeong and Nam, \{Min Hee\} and Yun, \{Doh Won\} and Eun, \{Moo Young\}",

year = "2005",

doi = "10.1081/PB-200065652",

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volume = "35",

pages = "257--262",

journal = "Preparative Biochemistry and Biotechnology",

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T1 - Rapid and simple procedure for homogenizing leaf tissues suitable for mini-midi-scale DNA extraction in rice

AU - Yi, Gihwan

AU - Choi, Jun Ho

AU - Lee, Jong Hee

AU - Jeong, Unggi

AU - Nam, Min Hee

AU - Yun, Doh Won

AU - Eun, Moo Young

PY - 2005

Y1 - 2005

N2 - We describe a rapid and simple procedure for homogenizing leaf samples suitable for mini/midi-scale DNA preparation in rice. The methods used tungsten carbide beads and general vortexer for homogenizing leaf samples. In general, two samples can be ground completely within 11.3 ± 1.5 sec at one time. Up to 20 samples can be ground at a time using a vortexer attachment. The yields of the DNA ranged from 2.2 to 7.6 μg from 25-150 mg of young fresh leaf tissue. The quality and quantity of DNA was compatible for most of PCR work and RFLP analysis.

AB - We describe a rapid and simple procedure for homogenizing leaf samples suitable for mini/midi-scale DNA preparation in rice. The methods used tungsten carbide beads and general vortexer for homogenizing leaf samples. In general, two samples can be ground completely within 11.3 ± 1.5 sec at one time. Up to 20 samples can be ground at a time using a vortexer attachment. The yields of the DNA ranged from 2.2 to 7.6 μg from 25-150 mg of young fresh leaf tissue. The quality and quantity of DNA was compatible for most of PCR work and RFLP analysis.

KW - DNA

KW - Mini/midi-preparation

KW - Tungsten beads

KW - Vortexer

UR - https://www.scopus.com/pages/publications/24644442222

U2 - 10.1081/PB-200065652

DO - 10.1081/PB-200065652

M3 - Article

C2 - 16109637

AN - SCOPUS:24644442222

SN - 1082-6068

VL - 35

SP - 257

EP - 262

JO - Preparative Biochemistry and Biotechnology

JF - Preparative Biochemistry and Biotechnology

IS - 3

ER -

Rapid and simple procedure for homogenizing leaf tissues suitable for mini-midi-scale DNA extraction in rice

Abstract

Keywords

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