Abstract
Bacillus sp. KYJ 963,a local isolate, produced an extracellular amylase with Mr = 59 kDa. The amylase was easily purified by adsorption on soluble starch. The analyses of TLC and N-terminal amino acid sequence from the purified protein revealed that the enzyme was a novel β-amylase which could not hydrolyze maltose or α-cyclodextrin and its N-terminal amino acid sequence was A-V-N-G-Q-S-F-N-S-N-Y-K-T-Y-K-.
Original language | English |
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Pages (from-to) | 1435-1438 |
Number of pages | 4 |
Journal | Biotechnology Letters |
Volume | 23 |
Issue number | 17 |
DOIs | |
State | Published - 2001 |
Keywords
- Anchovy-jeot
- Bacillus sp. KYJ 963
- Extracellular β-amylase
- Starch adsorption