TY - JOUR
T1 - Rapid biodegradation of chlorpyrifos by plant growth-promoting psychrophilic Shewanella sp. BT05
T2 - An eco-friendly approach to clean up pesticide-contaminated environment
AU - Govarthanan, M.
AU - Ameen, Fuad
AU - Kamala-Kannan, S.
AU - Selvankumar, T.
AU - Almansob, A.
AU - Alwakeel, S. S.
AU - Kim, Woong
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/5
Y1 - 2020/5
N2 - The present study explores the rapid chlopyrifos (CPs) biodegradation potential of plant growth promoting (PGP) psychrophilic bacteria isolated from brackish water by enrichment culture technique. Based on biochemical tests and 16 S rDNA sequencing the isolate was identified as Shewanella sp. The isolate Shewanella BT05 showed significant growth rate in various concentrations of (10–50 mg/L) CPs. The isolate produced plant growth promoting factors, IAA (20.8 ± 1.2 and 15.4 ± 1.0 μg/mL) and siderophores (60.67 ± 1.2 and 57.5 ± 0.9%) in the absence and presence of CPs. Further, the isolate BT05 solublized phosphate (16.5 ± 1.0 and 12.0 ± 1.0 mm in size respectively), and produce hydrogen cyanide (excellent and moderate) in the presence and absence of CPs. The isolate BT05 degraded 94.3, 91.8, 87.9, 82.6, and 80.5% of CPs at 10, 20, 30, 40, and 50 mg/L, respectively, within 24 h. Further, the media conditions were optimized for enhanced CPs removal and observed 93% removal in the presence of 3.5% glucose in pH 7.0 at 32.5 °C. Fourier-transform infrared spectroscopy and high-performance liquid chromatography results indicated the role for Shewanella BT05 in the biomineralization of CPs. The results suggested the isolate BT05 could be used for CPs removal as well as PGP activity in contaminated soil.
AB - The present study explores the rapid chlopyrifos (CPs) biodegradation potential of plant growth promoting (PGP) psychrophilic bacteria isolated from brackish water by enrichment culture technique. Based on biochemical tests and 16 S rDNA sequencing the isolate was identified as Shewanella sp. The isolate Shewanella BT05 showed significant growth rate in various concentrations of (10–50 mg/L) CPs. The isolate produced plant growth promoting factors, IAA (20.8 ± 1.2 and 15.4 ± 1.0 μg/mL) and siderophores (60.67 ± 1.2 and 57.5 ± 0.9%) in the absence and presence of CPs. Further, the isolate BT05 solublized phosphate (16.5 ± 1.0 and 12.0 ± 1.0 mm in size respectively), and produce hydrogen cyanide (excellent and moderate) in the presence and absence of CPs. The isolate BT05 degraded 94.3, 91.8, 87.9, 82.6, and 80.5% of CPs at 10, 20, 30, 40, and 50 mg/L, respectively, within 24 h. Further, the media conditions were optimized for enhanced CPs removal and observed 93% removal in the presence of 3.5% glucose in pH 7.0 at 32.5 °C. Fourier-transform infrared spectroscopy and high-performance liquid chromatography results indicated the role for Shewanella BT05 in the biomineralization of CPs. The results suggested the isolate BT05 could be used for CPs removal as well as PGP activity in contaminated soil.
KW - Biodegradation
KW - Chlorpyrifos
KW - HPLC
KW - Pesticide
KW - Plant growth promoting activity
UR - http://www.scopus.com/inward/record.url?scp=85078562602&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2020.125948
DO - 10.1016/j.chemosphere.2020.125948
M3 - Article
C2 - 32069723
AN - SCOPUS:85078562602
SN - 0045-6535
VL - 247
JO - Chemosphere
JF - Chemosphere
M1 - 125948
ER -