Rapid detection of Yersinia enterocolitica using a single–walled carbon nanotube-based biosensor for Kimchi product

The goal of this research was to develop a rapid single-walled carbon nanotube (SWCNT)-based biosensor method and to employ it to a Kimchi product for Yersinia enterocolitica detection. A SWCNT-based biosensor was fabricated with SWCNTs immobilized with anti-Yersinia antibody (pAbs) through hybridization of 1-pyrenebutanoic acid succinimidyl ester (PBASE) as a linker. The resistance difference (ΔR) was calculated via linear sweep voltammetry (LSV) using a potentiostat. The specific binding of pAbs to Y. enterocolitica was confirmed by indirect ELISA. The bacteria isolated from baek Kimchi products were identified using 16S rDNA sequencing. The ΔR for Y. enterocolitica was significantly increased by the fabricated SWCNT-based biosensor (p < 0.05). The indirect ELISA confirmed that the biosensor immobilized with pAbs was specific enough to detect Y. enterocolitica among the tested gram-negative bacteria. The optimim concentration range for the SWCNT-based biosensor was 10⁶ to 10⁴ CFU/mL, and the limit of detection (LOD) of the SWCNT-based biosensor was determined to be 10⁴ CFU/mL of Y. enterocolitica in both phosphate buffer solution (PBS) and Kimchi product.