Rapid engineering of foot-and-mouth disease vaccine and challenge viruses

Seo Yong Lee; Yeo Joo Lee; Rae Hyung Kim; Jeong Nam Park; Min Eun Park; Mi Kyeong Ko; Joo Hyung Choi; Jia Qi Chu; Kwang Nyeong Lee; Su Mi Kim; Dongseob Tark; Hyang Sim Lee; Young Joon Ko; Min Goo Seo; Jung Won Park; Byounghan Kim; Myoung Heon Lee; Jong Soo Lee; Jong Hyeon Park

doi:10.1128/JVI.00155-17

Rapid engineering of foot-and-mouth disease vaccine and challenge viruses

Seo Yong Lee, Yeo Joo Lee, Rae Hyung Kim, Jeong Nam Park, Min Eun Park, Mi Kyeong Ko, Joo Hyung Choi, Jia Qi Chu, Kwang Nyeong Lee, Su Mi Kim, Dongseob Tark, Hyang Sim Lee, Young Joon Ko, Min Goo Seo, Jung Won Park, Byounghan Kim, Myoung Heon Lee, Jong Soo Lee, Jong Hyeon Park

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

There are seven antigenically distinct serotypes of foot-and-mouth disease virus (FMDV), each of which has intratypic variants. In the present study, we have developed methods to efficiently generate promising vaccines against seven serotypes or subtypes. The capsid-encoding gene (P1) of the vaccine strain O1/ Manisa/Turkey/69 was replaced with the amplified or synthetic genes from the O, A, Asia1, C, SAT1, SAT2, and SAT3 serotypes. Viruses of the seven serotype were rescued successfully. Each chimeric FMDV with a replacement of P1 showed serotypespecific antigenicity and varied in terms of pathogenesis in pigs and mice. Vaccination of pigs with an experimental trivalent vaccine containing the inactivated recombinants based on the main serotypes O, A, and Asia1 effectively protected them from virus challenge. This technology could be a potential strategy for a customized vaccine with challenge tools to protect against epizootic disease caused by specific serotypes or subtypes of FMDV.

Original language	English
Article number	e00155-17
Journal	Journal of Virology
Volume	91
Issue number	16
DOIs	https://doi.org/10.1128/JVI.00155-17
State	Published - 1 Aug 2017

Keywords

Foot-and-mouth disease
Pathogenesis
Serotype
Synthetic vaccine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JVI.00155-17

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Lee, S. Y., Lee, Y. J., Kim, R. H., Park, J. N., Park, M. E., Ko, M. K., Choi, J. H., Chu, J. Q., Lee, K. N., Kim, S. M., Tark, D., Lee, H. S., Ko, Y. J., Seo, M. G., Park, J. W., Kim, B., Lee, M. H., Lee, J. S., & Park, J. H. (2017). Rapid engineering of foot-and-mouth disease vaccine and challenge viruses. Journal of Virology, 91(16), Article e00155-17. https://doi.org/10.1128/JVI.00155-17

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abstract = "There are seven antigenically distinct serotypes of foot-and-mouth disease virus (FMDV), each of which has intratypic variants. In the present study, we have developed methods to efficiently generate promising vaccines against seven serotypes or subtypes. The capsid-encoding gene (P1) of the vaccine strain O1/ Manisa/Turkey/69 was replaced with the amplified or synthetic genes from the O, A, Asia1, C, SAT1, SAT2, and SAT3 serotypes. Viruses of the seven serotype were rescued successfully. Each chimeric FMDV with a replacement of P1 showed serotypespecific antigenicity and varied in terms of pathogenesis in pigs and mice. Vaccination of pigs with an experimental trivalent vaccine containing the inactivated recombinants based on the main serotypes O, A, and Asia1 effectively protected them from virus challenge. This technology could be a potential strategy for a customized vaccine with challenge tools to protect against epizootic disease caused by specific serotypes or subtypes of FMDV.",

keywords = "Foot-and-mouth disease, Pathogenesis, Serotype, Synthetic vaccine",

author = "Lee, {Seo Yong} and Lee, {Yeo Joo} and Kim, {Rae Hyung} and Park, {Jeong Nam} and Park, {Min Eun} and Ko, {Mi Kyeong} and Choi, {Joo Hyung} and Chu, {Jia Qi} and Lee, {Kwang Nyeong} and Kim, {Su Mi} and Dongseob Tark and Lee, {Hyang Sim} and Ko, {Young Joon} and Seo, {Min Goo} and Park, {Jung Won} and Byounghan Kim and Lee, {Myoung Heon} and Lee, {Jong Soo} and Park, {Jong Hyeon}",

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AU - Choi, Joo Hyung

AU - Chu, Jia Qi

AU - Lee, Kwang Nyeong

AU - Kim, Su Mi

AU - Tark, Dongseob

AU - Lee, Hyang Sim

AU - Ko, Young Joon

AU - Seo, Min Goo

AU - Park, Jung Won

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AU - Lee, Myoung Heon

AU - Lee, Jong Soo

AU - Park, Jong Hyeon

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N2 - There are seven antigenically distinct serotypes of foot-and-mouth disease virus (FMDV), each of which has intratypic variants. In the present study, we have developed methods to efficiently generate promising vaccines against seven serotypes or subtypes. The capsid-encoding gene (P1) of the vaccine strain O1/ Manisa/Turkey/69 was replaced with the amplified or synthetic genes from the O, A, Asia1, C, SAT1, SAT2, and SAT3 serotypes. Viruses of the seven serotype were rescued successfully. Each chimeric FMDV with a replacement of P1 showed serotypespecific antigenicity and varied in terms of pathogenesis in pigs and mice. Vaccination of pigs with an experimental trivalent vaccine containing the inactivated recombinants based on the main serotypes O, A, and Asia1 effectively protected them from virus challenge. This technology could be a potential strategy for a customized vaccine with challenge tools to protect against epizootic disease caused by specific serotypes or subtypes of FMDV.

AB - There are seven antigenically distinct serotypes of foot-and-mouth disease virus (FMDV), each of which has intratypic variants. In the present study, we have developed methods to efficiently generate promising vaccines against seven serotypes or subtypes. The capsid-encoding gene (P1) of the vaccine strain O1/ Manisa/Turkey/69 was replaced with the amplified or synthetic genes from the O, A, Asia1, C, SAT1, SAT2, and SAT3 serotypes. Viruses of the seven serotype were rescued successfully. Each chimeric FMDV with a replacement of P1 showed serotypespecific antigenicity and varied in terms of pathogenesis in pigs and mice. Vaccination of pigs with an experimental trivalent vaccine containing the inactivated recombinants based on the main serotypes O, A, and Asia1 effectively protected them from virus challenge. This technology could be a potential strategy for a customized vaccine with challenge tools to protect against epizootic disease caused by specific serotypes or subtypes of FMDV.

KW - Foot-and-mouth disease

KW - Pathogenesis

KW - Serotype

KW - Synthetic vaccine

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Rapid engineering of foot-and-mouth disease vaccine and challenge viruses

Abstract

Keywords

UN SDGs

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