Abstract
Alkaline cellulolytic enzymes from cultured medium of Coprinus cinereus 2249 were purified with gel and ion-exchange chromatography and characteristics of those enzyme proteins were investigated. A fiber length distribution and a crystallinity of cellulose and sugar composition of enzyme treated Mixed Office Wastepaper(MOW) and Unbleached Kraft Pulp(UKP) were analysed. The conclusion could summarized as follows; Alkaline and acidic, endo- and exo-glucanases were purified from cultured medium of Coprinus cinereus 2249. The approximate molecular weight of alkaline endo-glucanase was 42 kDa, and also that of alkaline exo-glucanase was 50 kDa. A fiber length distribution and a crystallization of cellulose and sugar composition of enzyme treated MOW and UKP were not so much changed with original paper and pulp.
| Original language | English |
|---|---|
| Pages (from-to) | 24-29 |
| Number of pages | 6 |
| Journal | Palpu Chongi Gisul/Journal of Korea Technical Association of the Pulp and Paper Industry |
| Volume | 36 |
| Issue number | 1 |
| State | Published - 2004 |
Keywords
- Bleaching
- Cellulose
- Deinking
- Enzyme
- Recycling
- Sticky