Recycling of waste paper with alkaline cellulolytic enzymes(I) - Preparation and characteristics of cullulolytic enzymes

Alkaline cellulolytic enzymes are prepared from Coprinus cinereus 2249. Recovery method of enzyme protein from cultured medium and effect factors on enzyme activity of protein were investigated. The results could summarized as follows, 1 Amount of enzyme protein from cultured medium was highest in incubation with shaking and addition of skim milk. 2 Protein from cultured medium was alkaline enzymatic protein which shows the highest activity at pH 9.0. 3 The most effective recovery method of enzyme protein was the precipitation of protein by addition of cultured medium of protein in ethanol. 4 The enzyme activity was enhanced by tween-80 and decreased with Al ₂(SO₄)₃, H₂O₂ et al, and was little changed with metal ions except Hg⁺⁺.