Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3

Hyun Jin Lee; Chul Hong Park; Hyung U. Son; Jin Chul Heo; Sung Hee Nam; Kwang Gil Lee; Joo Hong Yeo; Cheol Sik Yoon; Jong Myeung Kim; Yong Kyu Shin; Si Oh Kim; Sang Han Lee

doi:10.3892/etm.2011.214

Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3

Hyun Jin Lee, Chul Hong Park, Hyung U. Son, Jin Chul Heo, Sung Hee Nam, Kwang Gil Lee, Joo Hong Yeo, Cheol Sik Yoon, Jong Myeung Kim, Yong Kyu Shin, Si Oh Kim, Sang Han Lee

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The aim of the present study was to investigate the anti-tumor effects of a culture filtrate of Paecilomyces farinosus J3. Various anti-tumor assays using B16 melanoma cells were carried out. Paecilomyces farinosus J3 significantly decreased the wound healing capability, invasiveness and angiogenic activity, which was confirmed by wound healing, human umbilical vein endothelial cell and invasion assays. Paecilomyces farinosus J3 strongly inhibited cell migration, tube formation and the angiogenic process in a concentration-dependent manner. Zymographic analysis also indicated a reduced expression of matrix metalloproteinase-9 (MMP-9), a 92-kDa gelatinase. Taken together, the results indicate that the anti-tumor activities of Paecilomyces farinosus J3 originate from the reduction of MMP-9 expression in B16F10 cells.

Original language	English
Pages (from-to)	357-362
Number of pages	6
Journal	Experimental and Therapeutic Medicine
Volume	2
Issue number	2
DOIs	https://doi.org/10.3892/etm.2011.214
State	Published - Mar 2011

Keywords

Anti-tumor activity
Culture filtrate
Invasion
Matrix metalloproteinase-9
Paecilomyces farinosus J3
Wound healing

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3892/etm.2011.214

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title = "Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3",

abstract = "The aim of the present study was to investigate the anti-tumor effects of a culture filtrate of Paecilomyces farinosus J3. Various anti-tumor assays using B16 melanoma cells were carried out. Paecilomyces farinosus J3 significantly decreased the wound healing capability, invasiveness and angiogenic activity, which was confirmed by wound healing, human umbilical vein endothelial cell and invasion assays. Paecilomyces farinosus J3 strongly inhibited cell migration, tube formation and the angiogenic process in a concentration-dependent manner. Zymographic analysis also indicated a reduced expression of matrix metalloproteinase-9 (MMP-9), a 92-kDa gelatinase. Taken together, the results indicate that the anti-tumor activities of Paecilomyces farinosus J3 originate from the reduction of MMP-9 expression in B16F10 cells.",

keywords = "Anti-tumor activity, Culture filtrate, Invasion, Matrix metalloproteinase-9, Paecilomyces farinosus J3, Wound healing",

author = "Lee, {Hyun Jin} and Park, {Chul Hong} and Son, {Hyung U.} and Heo, {Jin Chul} and Nam, {Sung Hee} and Lee, {Kwang Gil} and Yeo, {Joo Hong} and Yoon, {Cheol Sik} and Kim, {Jong Myeung} and Shin, {Yong Kyu} and Kim, {Si Oh} and Lee, {Sang Han}",

year = "2011",

month = mar,

doi = "10.3892/etm.2011.214",

language = "English",

volume = "2",

pages = "357--362",

journal = "Experimental and Therapeutic Medicine",

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AU - Park, Chul Hong

AU - Son, Hyung U.

AU - Heo, Jin Chul

AU - Nam, Sung Hee

AU - Lee, Kwang Gil

AU - Yeo, Joo Hong

AU - Yoon, Cheol Sik

AU - Kim, Jong Myeung

AU - Shin, Yong Kyu

AU - Kim, Si Oh

AU - Lee, Sang Han

PY - 2011/3

Y1 - 2011/3

N2 - The aim of the present study was to investigate the anti-tumor effects of a culture filtrate of Paecilomyces farinosus J3. Various anti-tumor assays using B16 melanoma cells were carried out. Paecilomyces farinosus J3 significantly decreased the wound healing capability, invasiveness and angiogenic activity, which was confirmed by wound healing, human umbilical vein endothelial cell and invasion assays. Paecilomyces farinosus J3 strongly inhibited cell migration, tube formation and the angiogenic process in a concentration-dependent manner. Zymographic analysis also indicated a reduced expression of matrix metalloproteinase-9 (MMP-9), a 92-kDa gelatinase. Taken together, the results indicate that the anti-tumor activities of Paecilomyces farinosus J3 originate from the reduction of MMP-9 expression in B16F10 cells.

AB - The aim of the present study was to investigate the anti-tumor effects of a culture filtrate of Paecilomyces farinosus J3. Various anti-tumor assays using B16 melanoma cells were carried out. Paecilomyces farinosus J3 significantly decreased the wound healing capability, invasiveness and angiogenic activity, which was confirmed by wound healing, human umbilical vein endothelial cell and invasion assays. Paecilomyces farinosus J3 strongly inhibited cell migration, tube formation and the angiogenic process in a concentration-dependent manner. Zymographic analysis also indicated a reduced expression of matrix metalloproteinase-9 (MMP-9), a 92-kDa gelatinase. Taken together, the results indicate that the anti-tumor activities of Paecilomyces farinosus J3 originate from the reduction of MMP-9 expression in B16F10 cells.

KW - Anti-tumor activity

KW - Culture filtrate

KW - Invasion

KW - Matrix metalloproteinase-9

KW - Paecilomyces farinosus J3

KW - Wound healing

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SN - 1792-0981

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ER -

Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3

Abstract

Keywords

UN SDGs

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