Refining hot-water extracted silk sericin by ethanol-induced precipitation

Hanjin Oh; Ji Young Lee; Moo Kon Kim; In Chul Um; Ki Hoon Lee

doi:10.1016/j.ijbiomac.2010.09.008

Refining hot-water extracted silk sericin by ethanol-induced precipitation

Hanjin Oh, Ji Young Lee, Moo Kon Kim, In Chul Um, Ki Hoon Lee

Department of Biofibers and Biomaterials Science

Seoul National University

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

In order to improve some inherent disadvantages in the mechanical properties of silk sericin (SS), we prepared ethanol-precipitated sericin (EpSS) by adding ethanol into hot-water extracted sericin (HS) solution. EpSS had higher viscosity compared to HS and it was due to the differences in their molecular weight distribution (MWD). The different MWD of EpSS was due to the different solubility of sericin molecules at various concentrations of ethanol, and the amino acid composition of EpSS showed that the more hydrophobic sericin is precipitated more readily in the presence of ethanol. The secondary structure of sericin is also changed from a random coil to β-sheet structure when the amount of ethanol added is high enough. The DSC analysis also revealed that EpSSs has a more compact structure. Finally, when beads were prepared from EpSS, they had enhanced compressive strength compared to those from HS.

Original language	English
Pages (from-to)	32-37
Number of pages	6
Journal	International Journal of Biological Macromolecules
Volume	48
Issue number	1
DOIs	https://doi.org/10.1016/j.ijbiomac.2010.09.008
State	Published - 1 Jan 2011

Keywords

Bombyx mori
Protein-based material
Sericin
Silk

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10.1016/j.ijbiomac.2010.09.008

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title = "Refining hot-water extracted silk sericin by ethanol-induced precipitation",

abstract = "In order to improve some inherent disadvantages in the mechanical properties of silk sericin (SS), we prepared ethanol-precipitated sericin (EpSS) by adding ethanol into hot-water extracted sericin (HS) solution. EpSS had higher viscosity compared to HS and it was due to the differences in their molecular weight distribution (MWD). The different MWD of EpSS was due to the different solubility of sericin molecules at various concentrations of ethanol, and the amino acid composition of EpSS showed that the more hydrophobic sericin is precipitated more readily in the presence of ethanol. The secondary structure of sericin is also changed from a random coil to β-sheet structure when the amount of ethanol added is high enough. The DSC analysis also revealed that EpSSs has a more compact structure. Finally, when beads were prepared from EpSS, they had enhanced compressive strength compared to those from HS.",

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AU - Oh, Hanjin

AU - Lee, Ji Young

AU - Kim, Moo Kon

AU - Um, In Chul

AU - Lee, Ki Hoon

PY - 2011/1/1

Y1 - 2011/1/1

N2 - In order to improve some inherent disadvantages in the mechanical properties of silk sericin (SS), we prepared ethanol-precipitated sericin (EpSS) by adding ethanol into hot-water extracted sericin (HS) solution. EpSS had higher viscosity compared to HS and it was due to the differences in their molecular weight distribution (MWD). The different MWD of EpSS was due to the different solubility of sericin molecules at various concentrations of ethanol, and the amino acid composition of EpSS showed that the more hydrophobic sericin is precipitated more readily in the presence of ethanol. The secondary structure of sericin is also changed from a random coil to β-sheet structure when the amount of ethanol added is high enough. The DSC analysis also revealed that EpSSs has a more compact structure. Finally, when beads were prepared from EpSS, they had enhanced compressive strength compared to those from HS.

AB - In order to improve some inherent disadvantages in the mechanical properties of silk sericin (SS), we prepared ethanol-precipitated sericin (EpSS) by adding ethanol into hot-water extracted sericin (HS) solution. EpSS had higher viscosity compared to HS and it was due to the differences in their molecular weight distribution (MWD). The different MWD of EpSS was due to the different solubility of sericin molecules at various concentrations of ethanol, and the amino acid composition of EpSS showed that the more hydrophobic sericin is precipitated more readily in the presence of ethanol. The secondary structure of sericin is also changed from a random coil to β-sheet structure when the amount of ethanol added is high enough. The DSC analysis also revealed that EpSSs has a more compact structure. Finally, when beads were prepared from EpSS, they had enhanced compressive strength compared to those from HS.

KW - Bombyx mori

KW - Protein-based material

KW - Sericin

KW - Silk

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Refining hot-water extracted silk sericin by ethanol-induced precipitation

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Keywords

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