Abstract
Resveratrol (3,4′,5 tri-hydroxystilbene), a naturally occurring polyphenolic compound highly enriched in grapes and red wine, has been shown to induce anti-proliferation and apoptosis of human cancer cell lines. Resveratrol-induced dose-dependent apoptotic cell death in colon carcinoma cells, was measured by FACS analysis. Treatment of HT29 human colon carcinoma cells with resveratrol was found to induce a number of signature ER stress markers; phosphorylation of eukaryotic initiation factor-2α (eIF-2α), ER stress-specific XBP1 splicing and CCAAT/enhancer-binding protein-homologous protein (CHOP). In addition, resveratrol induced up-regulation of glucose-regulated protein (GRP)-78, suggesting the induction of ER stress. Furthermore, the inhibition of caspase-4 activity by z-LEVD-fmk significantly reduced resveratrol-induced apoptosis. Taken together, the present study therefore provides strong evidence to support an important role of ER stress response in mediating the resveratrol-induced apoptosis.
Original language | English |
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Pages (from-to) | 1269-1273 |
Number of pages | 5 |
Journal | Oncology Reports |
Volume | 18 |
Issue number | 5 |
DOIs | |
State | Published - Nov 2007 |
Keywords
- Apoptosis
- CCAAT/enhancer-binding protein-homologous protein
- Endoplasmic reticulum stress
- Resveratrol
- Unfolded protein response