Resveratrol induces pro-apoptotic endoplasmic reticulum stress in human colon cancer cells

Jong Wook Park, Kyung Jin Woo, Jung Tae Lee, Jun Hee Lim, Tae Jin Lee, Sang Hyun Kim, Yung Hyun Choi, Taeg Kyu Kwon

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Resveratrol (3,4′,5 tri-hydroxystilbene), a naturally occurring polyphenolic compound highly enriched in grapes and red wine, has been shown to induce anti-proliferation and apoptosis of human cancer cell lines. Resveratrol-induced dose-dependent apoptotic cell death in colon carcinoma cells, was measured by FACS analysis. Treatment of HT29 human colon carcinoma cells with resveratrol was found to induce a number of signature ER stress markers; phosphorylation of eukaryotic initiation factor-2α (eIF-2α), ER stress-specific XBP1 splicing and CCAAT/enhancer-binding protein-homologous protein (CHOP). In addition, resveratrol induced up-regulation of glucose-regulated protein (GRP)-78, suggesting the induction of ER stress. Furthermore, the inhibition of caspase-4 activity by z-LEVD-fmk significantly reduced resveratrol-induced apoptosis. Taken together, the present study therefore provides strong evidence to support an important role of ER stress response in mediating the resveratrol-induced apoptosis.

Original languageEnglish
Pages (from-to)1269-1273
Number of pages5
JournalOncology Reports
Volume18
Issue number5
DOIs
StatePublished - Nov 2007

Keywords

  • Apoptosis
  • CCAAT/enhancer-binding protein-homologous protein
  • Endoplasmic reticulum stress
  • Resveratrol
  • Unfolded protein response

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