Retinoic acid induces hypersegmentation and enhances cytotoxicity of neutrophils against cancer cells

Sanjeeb Shrestha, Shin Yeong Kim, Young Jin Yun, Jun Kyu Kim, Jae Man Lee, Minsang Shin, Dong Keun Song, Chang Won Hong

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Hypersegmentation of nuclei is considered a distinct characteristic of the antitumoral phenotype of neutrophils. Retinoic acid, a metabolite of retinol, reorganizes and induces segmentation of the nucleus during the differentiation of neutrophils. However, the role of retinoic acid in the phenotype polarization of neutrophils has not been fully established. Here, we investigated the effect of retinoic acid on phenotype polarization of neutrophils. Retinoic acid-induced the hypersegmentation of human neutrophils via retinoic acid receptors and mTOR pathways. Retinoic acid-induced hypersegmented neutrophils enhanced neutrophil extracellular traps (NETs) formation in response to phorbol-12-myristate 13-acetate (PMA) and fMLP (N-Formylmethionine-leucyl-phenylalanine) stimulation, and increased cytotoxicity against various tumor cells. Moreover, retinoic acid treatment attenuated tumor growth in a murine model of tumor. Taken together, these results suggests that retinoic acid induces the phenotype polarization of neutrophils to exert antitumor effects.

Original languageEnglish
Pages (from-to)24-29
Number of pages6
JournalImmunology Letters
Volume182
DOIs
StatePublished - 1 Feb 2017

Keywords

  • Cytotoxicity
  • Neutrophils
  • Phenotype
  • Polarization
  • Retinoic acid

Fingerprint

Dive into the research topics of 'Retinoic acid induces hypersegmentation and enhances cytotoxicity of neutrophils against cancer cells'. Together they form a unique fingerprint.

Cite this