RGD immobilized human demineralized dentin particles for bone autografting

The purpose of this work is to modify demineralized dentin particle (DDP) with cell adhesion peptide (Arg-Gly-Asp; RGD) for bone regeneration. DDP was prepared with EDTA chelating agent and proved cell proliferation and mineralization effect in vitro. FT-IR analysis showed that DDP contained rich amine group on the surface. Endogenous amine group was applied for immobilization of a cell adhesive peptide. DDP surface was modified with RGD peptide. In mouse calvaria defect model, RGD immobilized DDP showed osteoconduction. Tartrateresistant acid phosphatase (TRAP) staining showed that the implanted DDP was dissolved by osteoclasts. Von Kossa staining proved mineral deposition on the defected area. Therefore, these results suggested that the surface modified DDP can be used as a suitable bone autograft material.