Role of Heat Shock Factors in Stress-Induced Transcription: An Update

Heyoun Bunch, Stuart K. Calderwood

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations

Abstract

Heat shock proteins (HSP) are rapidly induced after proteotoxic stresses such as heat shock and accumulate at high concentrations in cells. HSP induction involves primarily a family of heat shock transcription factors (HSF) that bind the heat shock elements of the HSP genes and mediate transcription in trans. We discuss methods for the study of HSP binding to HSP promoters and the consequent increases in HSP gene expression in vitro and in vivo.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages25-38
Number of pages14
DOIs
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2693
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Binding
  • Chromatin immunoprecipitation
  • Factor
  • Heat
  • Heat shock protein
  • Nuclear run-on
  • Purification
  • Shock
  • Transcription

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