Rumex acetosella Inhibits Platelet Function via Impaired MAPK and Phosphoinositide 3-Kinase Signaling

Bo Ra Jeon; Muhammad Irfan; Seung Eun Lee; Jeong Hoon Lee; Man Hee Rhee

doi:10.1007/s11655-021-2873-0

Rumex acetosella Inhibits Platelet Function via Impaired MAPK and Phosphoinositide 3-Kinase Signaling

Bo Ra Jeon, Muhammad Irfan, Seung Eun Lee, Jeong Hoon Lee, Man Hee Rhee

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Objective: To examine the antiplatelet and antithrombotic activity of Rumex acetosella extract. Methods: Standard light aggregometry was used for platelet aggregation, intracellular calcium mobilization assessed using Fura-2/AM, granule secretion (ATP release) by luminometer, and fibrinogen binding to integrin α_IIbβ₃ detected using flow cytometry. Western blotting is carried out to determine the phosphorylation of mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt signaling. Results: Rumex acetosella displayed the ability to inhibit platelet aggregation, calcium mobilization, granule secretion, and fibrinogen binding to integrin α_IIbβ₃. Rumex acetosella has also down-regulated MAPK and PI3K/Akt phosphorylation (all P<0.01). Conclusion: Rumex acetosella extract exhibits antiplatelet activity via modulating GPVI signaling, and it may protect against the development of platelet-related cardiovascular diseases.

Original language	English
Pages (from-to)	802-808
Number of pages	7
Journal	Chinese Journal of Integrative Medicine
Volume	28
Issue number	9
DOIs	https://doi.org/10.1007/s11655-021-2873-0
State	Published - Sep 2022

Keywords

antiplatelet
ethnomedicine
integrin αβ
mitogen-activated protein kinase
Rumex acetosella L

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11655-021-2873-0

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@article{e79ae84d99b04921aca089b047bfc0b6,

title = "Rumex acetosella Inhibits Platelet Function via Impaired MAPK and Phosphoinositide 3-Kinase Signaling",

abstract = "Objective: To examine the antiplatelet and antithrombotic activity of Rumex acetosella extract. Methods: Standard light aggregometry was used for platelet aggregation, intracellular calcium mobilization assessed using Fura-2/AM, granule secretion (ATP release) by luminometer, and fibrinogen binding to integrin αIIbβ3 detected using flow cytometry. Western blotting is carried out to determine the phosphorylation of mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt signaling. Results: Rumex acetosella displayed the ability to inhibit platelet aggregation, calcium mobilization, granule secretion, and fibrinogen binding to integrin αIIbβ3. Rumex acetosella has also down-regulated MAPK and PI3K/Akt phosphorylation (all P<0.01). Conclusion: Rumex acetosella extract exhibits antiplatelet activity via modulating GPVI signaling, and it may protect against the development of platelet-related cardiovascular diseases.",

keywords = "antiplatelet, ethnomedicine, integrin αβ, mitogen-activated protein kinase, Rumex acetosella L",

author = "Jeon, {Bo Ra} and Muhammad Irfan and Lee, {Seung Eun} and Lee, {Jeong Hoon} and Rhee, {Man Hee}",

note = "Publisher Copyright: {\textcopyright} 2021, The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2022",

month = sep,

doi = "10.1007/s11655-021-2873-0",

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volume = "28",

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journal = "Chinese Journal of Integrative Medicine",

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T1 - Rumex acetosella Inhibits Platelet Function via Impaired MAPK and Phosphoinositide 3-Kinase Signaling

AU - Jeon, Bo Ra

AU - Irfan, Muhammad

AU - Lee, Seung Eun

AU - Lee, Jeong Hoon

AU - Rhee, Man Hee

PY - 2022/9

Y1 - 2022/9

N2 - Objective: To examine the antiplatelet and antithrombotic activity of Rumex acetosella extract. Methods: Standard light aggregometry was used for platelet aggregation, intracellular calcium mobilization assessed using Fura-2/AM, granule secretion (ATP release) by luminometer, and fibrinogen binding to integrin αIIbβ3 detected using flow cytometry. Western blotting is carried out to determine the phosphorylation of mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt signaling. Results: Rumex acetosella displayed the ability to inhibit platelet aggregation, calcium mobilization, granule secretion, and fibrinogen binding to integrin αIIbβ3. Rumex acetosella has also down-regulated MAPK and PI3K/Akt phosphorylation (all P<0.01). Conclusion: Rumex acetosella extract exhibits antiplatelet activity via modulating GPVI signaling, and it may protect against the development of platelet-related cardiovascular diseases.

AB - Objective: To examine the antiplatelet and antithrombotic activity of Rumex acetosella extract. Methods: Standard light aggregometry was used for platelet aggregation, intracellular calcium mobilization assessed using Fura-2/AM, granule secretion (ATP release) by luminometer, and fibrinogen binding to integrin αIIbβ3 detected using flow cytometry. Western blotting is carried out to determine the phosphorylation of mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K)/Akt signaling. Results: Rumex acetosella displayed the ability to inhibit platelet aggregation, calcium mobilization, granule secretion, and fibrinogen binding to integrin αIIbβ3. Rumex acetosella has also down-regulated MAPK and PI3K/Akt phosphorylation (all P<0.01). Conclusion: Rumex acetosella extract exhibits antiplatelet activity via modulating GPVI signaling, and it may protect against the development of platelet-related cardiovascular diseases.

KW - antiplatelet

KW - ethnomedicine

KW - integrin αβ

KW - mitogen-activated protein kinase

KW - Rumex acetosella L

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U2 - 10.1007/s11655-021-2873-0

DO - 10.1007/s11655-021-2873-0

M3 - Article

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AN - SCOPUS:85115241687

SN - 1672-0415

VL - 28

SP - 802

EP - 808

JO - Chinese Journal of Integrative Medicine

JF - Chinese Journal of Integrative Medicine

IS - 9

ER -

Rumex acetosella Inhibits Platelet Function via Impaired MAPK and Phosphoinositide 3-Kinase Signaling

Abstract

Keywords

UN SDGs

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