S-(-)-10,11-Dihydroxyfarnesoic acid methyl ester inhibits melanin synthesis in murine Melanocyte cells

Seung Hwa Baek, Jun Won Ahn, Sung Hee Nam, Cheol Sik Yoon, Jae Cheon Shin, Sang Han Lee

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The development of antimelanogenic agents is important for the prevention of serious aesthetic problems such as melasmas, freckles, age spots, and chloasmas. In the course of screening for melanin synthesis inhibitors, we found that the culture broth from an insect morphopathogenic fungus, Beauveria bassiana CS1029, exhibits potent antimelanogenic activity. We isolated and purified an active metabolite and identified it as S-(-)-10,11-dihydroxyfarnesoic acid methyl ester (dhFAME), an insect juvenile hormone. To address whether dhFAME inhibits melanin synthesis, we first measured the size of the melanin biosynthesis inhibition zone caused by dhFAME. dhFAME also showed inhibitory activity against mushroom tyrosinase in Melan-a cells. Intracellular, dose-dependent tyrosinase inhibition activity was also confirmed by zymography. In addition, we showed that dhFAME strongly inhibits melanin synthesis in Melan-a cells. Furthermore, we compared levels of TYR, TRP-1, TRP-2, MITF, and MC1R mRNA expression by reverse-transcription polymerase chain reaction and showed that treatment of Melan-a cells with 35 μM dhFAME led to an 11-fold decrease in TYR expression, a 6-fold decrease in TRP-2 expression, and a 5-fold decrease in MITF expression. Together, these results indicate that dhFAME is a potent inhibitor of melanin synthesis that can potentially be used for cosmetic biomaterial(s).

Original languageEnglish
Pages (from-to)12750-12763
Number of pages14
JournalInternational Journal of Molecular Sciences
Volume15
Issue number7
DOIs
StatePublished - 18 Jul 2014

Keywords

  • Dihydroxyfarnesoic acid
  • Inhibitor
  • Insect juvenile hormone
  • Melanin
  • Tyrosinase
  • Whitening

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