Serological and molecular detection of Anaplasma phagocytophilum in horses reared in Korea

S. H. Lee; K. T. Kim; S. H. Yun; E. Choi; G. H. Lee; Y. S. Park; K. H. Cho; S. Yi; O. D. Kwon; T. H. Kim; D. Kwak

doi:10.17221/8491-VETMED

Serological and molecular detection of Anaplasma phagocytophilum in horses reared in Korea

S. H. Lee, K. T. Kim, S. H. Yun, E. Choi, G. H. Lee, Y. S. Park, K. H. Cho, S. Yi, O. D. Kwon, T. H. Kim, D. Kwak

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The objective of this study was to investigate the nationwide prevalence of Anaplasma phagocytophilum in horses reared in Korea using a commercial ELISA kit and two different nested PCR (nPCR) analyses. Our analysis showed that 2.9% (true prevalence: 3.1%, 16/549) of the horses were seropositive by ELISA, and none were positive by the two nPCR analyses. Significant differences were observed in the ELISA results when the data were analysed according to breed and geographic region. In light of a recent case of human granulocytic anaplasmosis in Korea and the predicted increase in the number and distribution of ticks due to global warming, continuous monitoring and appropriate control programs for Anaplasma spp. and other tick-borne pathogens need to be established.

Original language	English
Pages (from-to)	533-538
Number of pages	6
Journal	Veterinarni Medicina
Volume	60
Issue number	10
DOIs	https://doi.org/10.17221/8491-VETMED
State	Published - 2015

Keywords

Anaplasmosis
ELISA
Equine
Korea
PCR

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10.17221/8491-VETMED

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title = "Serological and molecular detection of Anaplasma phagocytophilum in horses reared in Korea",

abstract = "The objective of this study was to investigate the nationwide prevalence of Anaplasma phagocytophilum in horses reared in Korea using a commercial ELISA kit and two different nested PCR (nPCR) analyses. Our analysis showed that 2.9% (true prevalence: 3.1%, 16/549) of the horses were seropositive by ELISA, and none were positive by the two nPCR analyses. Significant differences were observed in the ELISA results when the data were analysed according to breed and geographic region. In light of a recent case of human granulocytic anaplasmosis in Korea and the predicted increase in the number and distribution of ticks due to global warming, continuous monitoring and appropriate control programs for Anaplasma spp. and other tick-borne pathogens need to be established.",

keywords = "Anaplasmosis, ELISA, Equine, Korea, PCR",

author = "Lee, {S. H.} and Kim, {K. T.} and Yun, {S. H.} and E. Choi and Lee, {G. H.} and Park, {Y. S.} and Cho, {K. H.} and S. Yi and Kwon, {O. D.} and Kim, {T. H.} and D. Kwak",

year = "2015",

doi = "10.17221/8491-VETMED",

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volume = "60",

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journal = "Veterinarni Medicina",

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TY - JOUR

T1 - Serological and molecular detection of Anaplasma phagocytophilum in horses reared in Korea

AU - Lee, S. H.

AU - Kim, K. T.

AU - Yun, S. H.

AU - Choi, E.

AU - Lee, G. H.

AU - Park, Y. S.

AU - Cho, K. H.

AU - Yi, S.

AU - Kwon, O. D.

AU - Kim, T. H.

AU - Kwak, D.

PY - 2015

Y1 - 2015

N2 - The objective of this study was to investigate the nationwide prevalence of Anaplasma phagocytophilum in horses reared in Korea using a commercial ELISA kit and two different nested PCR (nPCR) analyses. Our analysis showed that 2.9% (true prevalence: 3.1%, 16/549) of the horses were seropositive by ELISA, and none were positive by the two nPCR analyses. Significant differences were observed in the ELISA results when the data were analysed according to breed and geographic region. In light of a recent case of human granulocytic anaplasmosis in Korea and the predicted increase in the number and distribution of ticks due to global warming, continuous monitoring and appropriate control programs for Anaplasma spp. and other tick-borne pathogens need to be established.

AB - The objective of this study was to investigate the nationwide prevalence of Anaplasma phagocytophilum in horses reared in Korea using a commercial ELISA kit and two different nested PCR (nPCR) analyses. Our analysis showed that 2.9% (true prevalence: 3.1%, 16/549) of the horses were seropositive by ELISA, and none were positive by the two nPCR analyses. Significant differences were observed in the ELISA results when the data were analysed according to breed and geographic region. In light of a recent case of human granulocytic anaplasmosis in Korea and the predicted increase in the number and distribution of ticks due to global warming, continuous monitoring and appropriate control programs for Anaplasma spp. and other tick-borne pathogens need to be established.

KW - Anaplasmosis

KW - ELISA

KW - Equine

KW - Korea

KW - PCR

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U2 - 10.17221/8491-VETMED

DO - 10.17221/8491-VETMED

M3 - Article

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SN - 0375-8427

VL - 60

SP - 533

EP - 538

JO - Veterinarni Medicina

JF - Veterinarni Medicina

IS - 10

ER -

Serological and molecular detection of Anaplasma phagocytophilum in horses reared in Korea

Abstract

Keywords

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