Signaling modulations of mir-206-3p in tooth morphogenesis

Sanjiv Neupane, Yam Prasad Aryal, Tae Young Kim, Chang Yeol Yeon, Chang Hyeon An, Ji Youn Kim, Hitoshi Yamamoto, Youngkyun Lee, Wern Joo Sohn, Jae Young Kim

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

MicroRNAs (miRNAs) are a class of naturally occurring small non-coding RNAs that post-transcriptionally regulate gene expression in organisms. Most mammalian miRNAs influence biological processes, including developmental changes, tissue morphogenesis and the maintenance of tissue identity, cell growth, differentiation, apoptosis, and metabolism. The miR-206-3p has been correlated with cancer; however, developmental roles of this miRNA are unclear. In this study, we examined the expression pattern and evaluated the developmental regulation of miR-206-3p during tooth morphogenesis using ex-vivo culture method. The expression pattern of miR-206-3p was examined in the epithelium and mesenchyme of developing tooth germ with stage-specific manners. Perturbation of the expression of miR-206-3p clearly altered expression patterns of dental-development–related signaling molecules, including Axin2, Bmp2, Fgf4, Lef1 and Shh. The gene expression complemented with change in cellular events including, apoptosis and proliferation which caused altered crown and pulp morphogenesis in renal-capsule–calcified teeth. Especially, mislocalization of β-Catenin and SMAD1/5/8 were observed alongside dramatic alterations in the expression patterns of Fgf4 and Shh. Overall, our data suggest that the miR-206-3p regulate the cellular physiology during tooth morphogenesis through modulation of the Wnt, Bmp, Fgf, and Shh signaling pathways to form proper tooth pulp and crown.

Original languageEnglish
Article number5251
Pages (from-to)1-15
Number of pages15
JournalInternational Journal of Molecular Sciences
Volume21
Issue number15
DOIs
StatePublished - Aug 2020

Keywords

  • Epigenetic regulation
  • Epithelial-mesenchymal interactions
  • Signaling regulations
  • Tooth crown formation

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