Silibinin inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes

Seul Gi Lee; Taeg Kyu Kwon; Ju Ock Nam

doi:10.4014/mbl.1610.10005

Silibinin inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes

Seul Gi Lee, Taeg Kyu Kwon, Ju Ock Nam

School of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

C/EBPβ and C/EBPδ are required for the initiation of adipogenesis and induce the expression of key adipogenic regulators, such as PPARγ and C/EBPα. In the present study, we have examined the effects of silibinin and its possible molecular mechanisms in regulating adipocyte differentiation and expression of C/EBPβ and C/EBPδ in the early stage of adipogenesis. Silibinin statistically significantly inhibits intracellular lipid accumulation and the mRNA expression of various genes involved at different stages during adipogenesis. Silibinin also suppresses expression of lipoprotein lipase (LPL), fatty acid binding protein 4 (AP2), and adiponectin in 3T3-L1 adipocytes. Thus, the anti-adipogenic effect of silibinin seems to originate from the ability to inhibit the expression of C/EBPβ and C/EBPδ. Furthermore, silibinin decreases cell viability for differentiation period and induces apoptotic cell death through capspase-3 activation.

Original language	English
Pages (from-to)	27-34
Number of pages	8
Journal	Microbiology and Biotechnology Letters
Volume	45
Issue number	1
DOIs	https://doi.org/10.4014/mbl.1610.10005
State	Published - Mar 2017

Keywords

3T3-L1 adipocytes
Anti-adipogenic
Obesity
Silibinin
Silybum marianum

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4014/mbl.1610.10005

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@article{845fa8828c524017a2657971a97d8399,

title = "Silibinin inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes",

abstract = "C/EBPβ and C/EBPδ are required for the initiation of adipogenesis and induce the expression of key adipogenic regulators, such as PPARγ and C/EBPα. In the present study, we have examined the effects of silibinin and its possible molecular mechanisms in regulating adipocyte differentiation and expression of C/EBPβ and C/EBPδ in the early stage of adipogenesis. Silibinin statistically significantly inhibits intracellular lipid accumulation and the mRNA expression of various genes involved at different stages during adipogenesis. Silibinin also suppresses expression of lipoprotein lipase (LPL), fatty acid binding protein 4 (AP2), and adiponectin in 3T3-L1 adipocytes. Thus, the anti-adipogenic effect of silibinin seems to originate from the ability to inhibit the expression of C/EBPβ and C/EBPδ. Furthermore, silibinin decreases cell viability for differentiation period and induces apoptotic cell death through capspase-3 activation.",

keywords = "3T3-L1 adipocytes, Anti-adipogenic, Obesity, Silibinin, Silybum marianum",

author = "Lee, \{Seul Gi\} and Kwon, \{Taeg Kyu\} and Nam, \{Ju Ock\}",

note = "Publisher Copyright: {\textcopyright} 2017, The Korean Society for Microbiology and Biotechnology.",

year = "2017",

month = mar,

doi = "10.4014/mbl.1610.10005",

language = "English",

volume = "45",

pages = "27--34",

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TY - JOUR

T1 - Silibinin inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes

AU - Lee, Seul Gi

AU - Kwon, Taeg Kyu

AU - Nam, Ju Ock

PY - 2017/3

Y1 - 2017/3

N2 - C/EBPβ and C/EBPδ are required for the initiation of adipogenesis and induce the expression of key adipogenic regulators, such as PPARγ and C/EBPα. In the present study, we have examined the effects of silibinin and its possible molecular mechanisms in regulating adipocyte differentiation and expression of C/EBPβ and C/EBPδ in the early stage of adipogenesis. Silibinin statistically significantly inhibits intracellular lipid accumulation and the mRNA expression of various genes involved at different stages during adipogenesis. Silibinin also suppresses expression of lipoprotein lipase (LPL), fatty acid binding protein 4 (AP2), and adiponectin in 3T3-L1 adipocytes. Thus, the anti-adipogenic effect of silibinin seems to originate from the ability to inhibit the expression of C/EBPβ and C/EBPδ. Furthermore, silibinin decreases cell viability for differentiation period and induces apoptotic cell death through capspase-3 activation.

AB - C/EBPβ and C/EBPδ are required for the initiation of adipogenesis and induce the expression of key adipogenic regulators, such as PPARγ and C/EBPα. In the present study, we have examined the effects of silibinin and its possible molecular mechanisms in regulating adipocyte differentiation and expression of C/EBPβ and C/EBPδ in the early stage of adipogenesis. Silibinin statistically significantly inhibits intracellular lipid accumulation and the mRNA expression of various genes involved at different stages during adipogenesis. Silibinin also suppresses expression of lipoprotein lipase (LPL), fatty acid binding protein 4 (AP2), and adiponectin in 3T3-L1 adipocytes. Thus, the anti-adipogenic effect of silibinin seems to originate from the ability to inhibit the expression of C/EBPβ and C/EBPδ. Furthermore, silibinin decreases cell viability for differentiation period and induces apoptotic cell death through capspase-3 activation.

KW - 3T3-L1 adipocytes

KW - Anti-adipogenic

KW - Obesity

KW - Silibinin

KW - Silybum marianum

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U2 - 10.4014/mbl.1610.10005

DO - 10.4014/mbl.1610.10005

M3 - Article

AN - SCOPUS:85018245522

SN - 1598-642X

VL - 45

SP - 27

EP - 34

JO - Microbiology and Biotechnology Letters

JF - Microbiology and Biotechnology Letters

IS - 1

ER -

Silibinin inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes

Abstract

Keywords

UN SDGs

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