Size-dependent free solution DNA electrophoresis in structured microfluidic systems

Thanh Tu Duong; Gyuman Kim; Robert Ros; Martin Streek; Friederike Schmid; J. ürgen Brugger; Dario Anselmetti; Alexandra Ros

doi:10.1016/S0167-9317(03)00153-9

Size-dependent free solution DNA electrophoresis in structured microfluidic systems

Thanh Tu Duong, Gyuman Kim, Robert Ros, Martin Streek, Friederike Schmid, J. ürgen Brugger, Dario Anselmetti, Alexandra Ros

Research output: Contribution to journal › Conference article › peer-review

55 Scopus citations

Abstract

A new concept based on microstructuring techniques is presented to achieve size-dependent electrophoretic migration of DNA in free solution. Topographically structured microfluidic channels with periodical cavities in the range of the radius of gyration of the tested DNA molecules (<3 μm) are produced by moulding of polydimethylsiloxane on a master wafer fabricated by contact lithography of SU-8 photoresist. The electrophoretic migration of single DNA molecules stained with the intercalator YOYO was investigated by real-time fluorescence video microscopy. It could be demonstrated for large DNA molecules that λ-DNA (48 kbp) always migrated significantly faster than T2-DNA (164 kbp) in the two tested microchannel layouts. Integration into a microdevice for DNA separation in free solution is now in progress.

Original language	English
Pages (from-to)	905-912
Number of pages	8
Journal	Microelectronic Engineering
Volume	67-68
DOIs	https://doi.org/10.1016/S0167-9317(03)00153-9
State	Published - Jun 2003
Event	Proceedings of the 28th International Conference on MNE - Lugano, Switzerland Duration: 16 Sep 2002 → 19 Sep 2002

Keywords

Contact lithography
DNA
Electrophoresis
Fluorescence microscopy
Microchip
Single molecule

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10.1016/S0167-9317(03)00153-9

Cite this

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title = "Size-dependent free solution DNA electrophoresis in structured microfluidic systems",

abstract = "A new concept based on microstructuring techniques is presented to achieve size-dependent electrophoretic migration of DNA in free solution. Topographically structured microfluidic channels with periodical cavities in the range of the radius of gyration of the tested DNA molecules (<3 μm) are produced by moulding of polydimethylsiloxane on a master wafer fabricated by contact lithography of SU-8 photoresist. The electrophoretic migration of single DNA molecules stained with the intercalator YOYO was investigated by real-time fluorescence video microscopy. It could be demonstrated for large DNA molecules that λ-DNA (48 kbp) always migrated significantly faster than T2-DNA (164 kbp) in the two tested microchannel layouts. Integration into a microdevice for DNA separation in free solution is now in progress.",

keywords = "Contact lithography, DNA, Electrophoresis, Fluorescence microscopy, Microchip, Single molecule",

author = "Duong, {Thanh Tu} and Gyuman Kim and Robert Ros and Martin Streek and Friederike Schmid and Brugger, {J. {\"u}rgen} and Dario Anselmetti and Alexandra Ros",

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doi = "10.1016/S0167-9317(03)00153-9",

language = "English",

volume = "67-68",

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journal = "Microelectronic Engineering",

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note = "Proceedings of the 28th International Conference on MNE ; Conference date: 16-09-2002 Through 19-09-2002",

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T1 - Size-dependent free solution DNA electrophoresis in structured microfluidic systems

AU - Duong, Thanh Tu

AU - Kim, Gyuman

AU - Ros, Robert

AU - Streek, Martin

AU - Schmid, Friederike

AU - Brugger, J. ürgen

AU - Anselmetti, Dario

AU - Ros, Alexandra

PY - 2003/6

Y1 - 2003/6

N2 - A new concept based on microstructuring techniques is presented to achieve size-dependent electrophoretic migration of DNA in free solution. Topographically structured microfluidic channels with periodical cavities in the range of the radius of gyration of the tested DNA molecules (<3 μm) are produced by moulding of polydimethylsiloxane on a master wafer fabricated by contact lithography of SU-8 photoresist. The electrophoretic migration of single DNA molecules stained with the intercalator YOYO was investigated by real-time fluorescence video microscopy. It could be demonstrated for large DNA molecules that λ-DNA (48 kbp) always migrated significantly faster than T2-DNA (164 kbp) in the two tested microchannel layouts. Integration into a microdevice for DNA separation in free solution is now in progress.

AB - A new concept based on microstructuring techniques is presented to achieve size-dependent electrophoretic migration of DNA in free solution. Topographically structured microfluidic channels with periodical cavities in the range of the radius of gyration of the tested DNA molecules (<3 μm) are produced by moulding of polydimethylsiloxane on a master wafer fabricated by contact lithography of SU-8 photoresist. The electrophoretic migration of single DNA molecules stained with the intercalator YOYO was investigated by real-time fluorescence video microscopy. It could be demonstrated for large DNA molecules that λ-DNA (48 kbp) always migrated significantly faster than T2-DNA (164 kbp) in the two tested microchannel layouts. Integration into a microdevice for DNA separation in free solution is now in progress.

KW - Contact lithography

KW - DNA

KW - Electrophoresis

KW - Fluorescence microscopy

KW - Microchip

KW - Single molecule

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U2 - 10.1016/S0167-9317(03)00153-9

DO - 10.1016/S0167-9317(03)00153-9

M3 - Conference article

AN - SCOPUS:0038358252

SN - 0167-9317

VL - 67-68

SP - 905

EP - 912

JO - Microelectronic Engineering

JF - Microelectronic Engineering

T2 - Proceedings of the 28th International Conference on MNE

Y2 - 16 September 2002 through 19 September 2002

ER -

Size-dependent free solution DNA electrophoresis in structured microfluidic systems

Abstract

Keywords

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