TY - JOUR
T1 - Soluble epoxide hydrolase inhibitory constituents from Selaginella tamariscina
AU - Kim, Jang Hoon
AU - Tai, Bui Huu
AU - Yang, Seo Young
AU - Kim, Ji Eun
AU - Kim, Sang Kyum
AU - Kim, Young Ho
N1 - Publisher Copyright:
© 2015 Korean Chemical Society, Seoul & Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015/1/20
Y1 - 2015/1/20
N2 - Anovel norneolignan, tamariscinol T (1), and six known compounds, protocatechuic acid (2), vanillic acid (3), caffeic acid (4), amentoflavone (5), 2,3-dihydroamentoflavone (6), and 2″ ,3″ -dihydroamentoflavone (7), were isolated from Selaginella tamariscina. Their structures were elucidated using 1D/2D NMR, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and infrared (IR), ultraviolet (UV), and circular dichroism (CD) spectroscopy. The effects of the isolated compounds on soluble epoxide hydrolase (sEH) activity were then evaluated. Compounds 5- 7 potently inhibited sEH activity with IC50 values of 24.3 ± 1.6, 5.4 ± 0.1, and 11.9 ± 0.1 μM, respectively. In addition, analysis of the kinetics of compounds 5-7 revealed that their inhibitory actions were noncompetitive, with inhibition constants (Ki) of 24.4 ± 1.2, 8.6 ± 0.4, and 5.3 ± 0.6 μM, respectively.
AB - Anovel norneolignan, tamariscinol T (1), and six known compounds, protocatechuic acid (2), vanillic acid (3), caffeic acid (4), amentoflavone (5), 2,3-dihydroamentoflavone (6), and 2″ ,3″ -dihydroamentoflavone (7), were isolated from Selaginella tamariscina. Their structures were elucidated using 1D/2D NMR, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and infrared (IR), ultraviolet (UV), and circular dichroism (CD) spectroscopy. The effects of the isolated compounds on soluble epoxide hydrolase (sEH) activity were then evaluated. Compounds 5- 7 potently inhibited sEH activity with IC50 values of 24.3 ± 1.6, 5.4 ± 0.1, and 11.9 ± 0.1 μM, respectively. In addition, analysis of the kinetics of compounds 5-7 revealed that their inhibitory actions were noncompetitive, with inhibition constants (Ki) of 24.4 ± 1.2, 8.6 ± 0.4, and 5.3 ± 0.6 μM, respectively.
KW - Non-competitive type
KW - Selaginella tamariscina
KW - Selaginellaceae
KW - Souble epoxide hydrolase
UR - http://www.scopus.com/inward/record.url?scp=84921913437&partnerID=8YFLogxK
U2 - 10.1002/bkcs.10068
DO - 10.1002/bkcs.10068
M3 - Article
AN - SCOPUS:84921913437
SN - 0253-2964
VL - 36
SP - 300
EP - 304
JO - Bulletin of the Korean Chemical Society
JF - Bulletin of the Korean Chemical Society
IS - 1
ER -