Somatic embryogenesis and plant regeneration from in-vitro-grown leaf explants of rose

C. K. Kim; J. Y. Oh; J. D. Chung; A. M. Burrell; D. H. Byrne

doi:10.21273/hortsci.39.6.1378

Somatic embryogenesis and plant regeneration from in-vitro-grown leaf explants of rose

C. K. Kim, J. Y. Oh, J. D. Chung, A. M. Burrell, D. H. Byrne

Department of Horticultural Science

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Somatic embryogenesis was initiated from in vitro-grown leaf explants of rose using an induction period of 4 weeks on MS basal medium supplemented with auxin followed by several subcultures on MS basal medium with cytokinin. '4 ^th of July' showed the highest regeneration frequency (24.4%) on 5.3 μM NAA followed by culture on medium containing 18.2 μM zeatin. 'Tournament of Roses' produced somatic embryos when cultured for 4 weeks on medium containing dicamba, 2.3 μM followed by three subcultures on medium containing 18.2 μM zeatin. Embryogenic callus matured on MS media containing 0.5 μM NAA, 6.8 μM zeatin, and 2.9 μM GA₃. Long-term cultures were established for both cultivars. Somatic embryos germinated on MS medium containing IBA and BA. Silver nitrate (58.8 μM) enhanced shoot formation and germination of somatic embryos. Plants derived from somatic embryos were acclimatized and successfully established in the greenhouse.

Original language	English
Pages (from-to)	1378-1380
Number of pages	3
Journal	Hortscience: A Publication of the American Society for Hortcultural Science
Volume	39
Issue number	6
DOIs	https://doi.org/10.21273/hortsci.39.6.1378
State	Published - Oct 2004

Keywords

Callus
Rosa
Silver nitrate

Access to Document

10.21273/hortsci.39.6.1378

Cite this

@article{2c10c73129714150887f467e190b87b8,

title = "Somatic embryogenesis and plant regeneration from in-vitro-grown leaf explants of rose",

abstract = "Somatic embryogenesis was initiated from in vitro-grown leaf explants of rose using an induction period of 4 weeks on MS basal medium supplemented with auxin followed by several subcultures on MS basal medium with cytokinin. '4 th of July' showed the highest regeneration frequency (24.4%) on 5.3 μM NAA followed by culture on medium containing 18.2 μM zeatin. 'Tournament of Roses' produced somatic embryos when cultured for 4 weeks on medium containing dicamba, 2.3 μM followed by three subcultures on medium containing 18.2 μM zeatin. Embryogenic callus matured on MS media containing 0.5 μM NAA, 6.8 μM zeatin, and 2.9 μM GA3. Long-term cultures were established for both cultivars. Somatic embryos germinated on MS medium containing IBA and BA. Silver nitrate (58.8 μM) enhanced shoot formation and germination of somatic embryos. Plants derived from somatic embryos were acclimatized and successfully established in the greenhouse.",

keywords = "Callus, Rosa, Silver nitrate",

author = "Kim, {C. K.} and Oh, {J. Y.} and Chung, {J. D.} and Burrell, {A. M.} and Byrne, {D. H.}",

year = "2004",

month = oct,

doi = "10.21273/hortsci.39.6.1378",

language = "English",

volume = "39",

pages = "1378--1380",

journal = "Hortscience: A Publication of the American Society for Hortcultural Science",

issn = "0018-5345",

publisher = "American Society for Horticultural Science",

number = "6",

}

TY - JOUR

T1 - Somatic embryogenesis and plant regeneration from in-vitro-grown leaf explants of rose

AU - Kim, C. K.

AU - Oh, J. Y.

AU - Chung, J. D.

AU - Burrell, A. M.

AU - Byrne, D. H.

PY - 2004/10

Y1 - 2004/10

N2 - Somatic embryogenesis was initiated from in vitro-grown leaf explants of rose using an induction period of 4 weeks on MS basal medium supplemented with auxin followed by several subcultures on MS basal medium with cytokinin. '4 th of July' showed the highest regeneration frequency (24.4%) on 5.3 μM NAA followed by culture on medium containing 18.2 μM zeatin. 'Tournament of Roses' produced somatic embryos when cultured for 4 weeks on medium containing dicamba, 2.3 μM followed by three subcultures on medium containing 18.2 μM zeatin. Embryogenic callus matured on MS media containing 0.5 μM NAA, 6.8 μM zeatin, and 2.9 μM GA3. Long-term cultures were established for both cultivars. Somatic embryos germinated on MS medium containing IBA and BA. Silver nitrate (58.8 μM) enhanced shoot formation and germination of somatic embryos. Plants derived from somatic embryos were acclimatized and successfully established in the greenhouse.

AB - Somatic embryogenesis was initiated from in vitro-grown leaf explants of rose using an induction period of 4 weeks on MS basal medium supplemented with auxin followed by several subcultures on MS basal medium with cytokinin. '4 th of July' showed the highest regeneration frequency (24.4%) on 5.3 μM NAA followed by culture on medium containing 18.2 μM zeatin. 'Tournament of Roses' produced somatic embryos when cultured for 4 weeks on medium containing dicamba, 2.3 μM followed by three subcultures on medium containing 18.2 μM zeatin. Embryogenic callus matured on MS media containing 0.5 μM NAA, 6.8 μM zeatin, and 2.9 μM GA3. Long-term cultures were established for both cultivars. Somatic embryos germinated on MS medium containing IBA and BA. Silver nitrate (58.8 μM) enhanced shoot formation and germination of somatic embryos. Plants derived from somatic embryos were acclimatized and successfully established in the greenhouse.

KW - Callus

KW - Rosa

KW - Silver nitrate

UR - http://www.scopus.com/inward/record.url?scp=5444234861&partnerID=8YFLogxK

U2 - 10.21273/hortsci.39.6.1378

DO - 10.21273/hortsci.39.6.1378

M3 - Article

AN - SCOPUS:5444234861

SN - 0018-5345

VL - 39

SP - 1378

EP - 1380

JO - Hortscience: A Publication of the American Society for Hortcultural Science

JF - Hortscience: A Publication of the American Society for Hortcultural Science

IS - 6

ER -

Somatic embryogenesis and plant regeneration from in-vitro-grown leaf explants of rose

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this