Spirosoma horti sp. Nov., isolated from apple orchard soil

A Gram-negative, motile by gliding, rod-shaped, aerobic bacterium, designated S7-3-19^T, was isolated from apple orchard soil in Gyeongsangnam-do Province, Republic of Korea, and characterized taxonomically by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain S7-3-19^T belonged to the family Cytophagaceae and was most closely related to Spirosoma linguale DSM 74^T (96.38 %), Spirosoma fluviale MSd3^T (96.38 %), Spirosoma pulveris JSH5-14^T (96.35 %) and Spirosoma radiotolerans DG5A^T (96.24 %). Chemotaxonomic characteristics supported the classification of strain S7-3-19^T within the genus Spirosoma. Summed feature 3 (C_{16: 1}!7c/C_{16: 1}!6c; 46.7 %) and C_{16: 1}!5c (23.8 %) were the major fatty acids. Phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified phospholipid and two unidentified lipids were the major polar lipids. Menaquinone with seven isoprene units was the predominant respiratory quinone. The G+C content of the genomic DNA of strain S7-3-19^T was 48.6 mol%. On the basis of its phenotypic properties, genotypic distinctiveness and chemotaxonomic features, strain S7-3-19^T represents a novel species of the genus Spirosoma, for which the name Spirosoma horti sp. nov. is proposed. The type strain is S7-3-19^T (=KCTC 52728^T=JCM 32131^T).