Spirosoma koreense sp. Nov., a species of the family cytophagaceae isolated from beach soil

A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J8-5^T, was isolated from beach soil on Jeju Island, Republic of Korea. The isolate grew at 10–37 °C (optimal 25 °C), pH 6–8 (optimal pH 7) and in the presence of 0–1% (w/v) NaCl. The results of comparative 16S rRNA gene sequence analysis indicated that strain 15J8-5^T represented a member of the family Cytophagaceae, phylum Bacteroidetes and was most closely related to Spirosoma knui 15J8-12^T (93.1% similarity), Spirosoma spitsbergense SPM-9^T (93.1 %) and Spirosoma endophyticum EX36^T (93.1 %). The G+C content of the genomic DNA of the novel strain was 48.0 mol%. The isolate contained menaquinone MK-7 as the predominant respiratory quinone, phosphatidylethanolamine as the major polar lipid, and summed feature 3 (C_{16: 1} ω7c/C_{16: 1}ω6c; 32.8 %), C_{16: 1}ω5c (24.8 %) and C_{16: 0} (11.7 %) as the major fatty acids, which supported the affiliation of strain 15J8-5T to the genus Spirosoma. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 15J8-5^T from members of the genus Spirosoma. Based on its phenotypic properties and phylogenetic distinctiveness, strain 15J8-5^T represents a novel species of the genus Spirosoma, for which the name Spirosoma koreense sp. nov. is proposed. The type strain is 15J8-5^T (=KCTC 52027^T=JCM 31992^T).