TY - JOUR
T1 - Spirosoma montaniterrae sp. nov., an ultraviolet and gamma radiation-resistant bacterium isolated from mountain soil
AU - Lee, Jae Jin
AU - Kang, Myung Suk
AU - Joo, Eun Sun
AU - Kim, Myung Kyum
AU - Im, Wan Taek
AU - Jung, Hee Young
AU - Srinivasan, Sathiyaraj
N1 - Publisher Copyright:
© 2015, The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.
PY - 2015/7/29
Y1 - 2015/7/29
N2 - A Gram-negative, yellow-pigmented, long-rod shaped bacterial strain designated DY10T was isolated from a soil sample collected at Mt. Deogyusan, Jeonbuk province, South Korea. Optimum growth observed at 30°C and pH 7. No growth was observed above 1% (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain DY10T belonged to the genus Spirosoma and was distantly related to Spirosoma arcticum R2–35T (91.0%), Spirosoma lingual DSM 74T (90.8%), Spirosoma endophyticum EX36T (90.7%), Spirosoma panaciterrae DSM 21099T (90.5%), Spirosoma rigui WPCB118T (90.2%), Spirosoma spitsbergense DSM 19989T (89.8%), Spirosoma luteum DSM 19990T (89.6%), Spirosoma oryzae RHs22T (89.6%), and Spirosoma radiotolerans DG5AT (89.1%). Strain DY10T showed resistance to gamma and ultraviolet radiation. The chemotaxonomic characteristics of strain DY10T were consistent with those of the genus Spirosoma, with the quinone system with MK-7 as the predominant menaquinone, iso-C15:0, C16:1ω5c, and summed feature3 (C16:1ω7c/C16:1ω6c), and phosphatidylethanolamine as the major polar lipid. The G+C content of the genomic DNA was 53.0 mol%. Differential phenotypic properties with the closely related type strains clearly distinguished strain DY10T from previously described members of the genus Spirosoma and represents a novel species in this genus, for which the name Spirosoma montaniterrae sp. nov. is proposed. The type strain is DY10T (=KCTC 23999T =KEMB 9004–162T =JCM 18492T).
AB - A Gram-negative, yellow-pigmented, long-rod shaped bacterial strain designated DY10T was isolated from a soil sample collected at Mt. Deogyusan, Jeonbuk province, South Korea. Optimum growth observed at 30°C and pH 7. No growth was observed above 1% (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain DY10T belonged to the genus Spirosoma and was distantly related to Spirosoma arcticum R2–35T (91.0%), Spirosoma lingual DSM 74T (90.8%), Spirosoma endophyticum EX36T (90.7%), Spirosoma panaciterrae DSM 21099T (90.5%), Spirosoma rigui WPCB118T (90.2%), Spirosoma spitsbergense DSM 19989T (89.8%), Spirosoma luteum DSM 19990T (89.6%), Spirosoma oryzae RHs22T (89.6%), and Spirosoma radiotolerans DG5AT (89.1%). Strain DY10T showed resistance to gamma and ultraviolet radiation. The chemotaxonomic characteristics of strain DY10T were consistent with those of the genus Spirosoma, with the quinone system with MK-7 as the predominant menaquinone, iso-C15:0, C16:1ω5c, and summed feature3 (C16:1ω7c/C16:1ω6c), and phosphatidylethanolamine as the major polar lipid. The G+C content of the genomic DNA was 53.0 mol%. Differential phenotypic properties with the closely related type strains clearly distinguished strain DY10T from previously described members of the genus Spirosoma and represents a novel species in this genus, for which the name Spirosoma montaniterrae sp. nov. is proposed. The type strain is DY10T (=KCTC 23999T =KEMB 9004–162T =JCM 18492T).
KW - Cytophagaceae
KW - radiation-resistant
KW - Spirosoma montaniterrae
KW - taxonomy
UR - http://www.scopus.com/inward/record.url?scp=84932633066&partnerID=8YFLogxK
U2 - 10.1007/s12275-015-5008-5
DO - 10.1007/s12275-015-5008-5
M3 - Article
C2 - 26115990
AN - SCOPUS:84932633066
SN - 1225-8873
VL - 53
SP - 429
EP - 434
JO - Journal of Microbiology
JF - Journal of Microbiology
IS - 7
ER -