Spirosoma montaniterrae sp. nov., an ultraviolet and gamma radiation-resistant bacterium isolated from mountain soil

A Gram-negative, yellow-pigmented, long-rod shaped bacterial strain designated DY10^T was isolated from a soil sample collected at Mt. Deogyusan, Jeonbuk province, South Korea. Optimum growth observed at 30°C and pH 7. No growth was observed above 1% (w/v) NaCl. Comparative 16S rRNA gene sequence analysis showed that strain DY10^T belonged to the genus Spirosoma and was distantly related to Spirosoma arcticum R2–35^T (91.0%), Spirosoma lingual DSM 74^T (90.8%), Spirosoma endophyticum EX36^T (90.7%), Spirosoma panaciterrae DSM 21099^T (90.5%), Spirosoma rigui WPCB118^T (90.2%), Spirosoma spitsbergense DSM 19989^T (89.8%), Spirosoma luteum DSM 19990^T (89.6%), Spirosoma oryzae RHs22^T (89.6%), and Spirosoma radiotolerans DG5A^T (89.1%). Strain DY10^T showed resistance to gamma and ultraviolet radiation. The chemotaxonomic characteristics of strain DY10^T were consistent with those of the genus Spirosoma, with the quinone system with MK-7 as the predominant menaquinone, iso-C_15:0, C_16:1ω5c, and summed feature3 (C_16:1ω7c/C_16:1ω6c), and phosphatidylethanolamine as the major polar lipid. The G+C content of the genomic DNA was 53.0 mol%. Differential phenotypic properties with the closely related type strains clearly distinguished strain DY10^T from previously described members of the genus Spirosoma and represents a novel species in this genus, for which the name Spirosoma montaniterrae sp. nov. is proposed. The type strain is DY10^T (=KCTC 23999^T =KEMB 9004–162^T =JCM 18492^T).