TY - JOUR
T1 - Spirosoma terrae sp. nov., Isolated from Soil from Jeju Island, Korea
AU - Ten, Leonid N.
AU - Okiria, Joseph
AU - Lee, Jae Jin
AU - Lee, Seung Yeol
AU - Park, Sangkyu
AU - Lee, Dae Sung
AU - Kang, In Kyu
AU - Kim, Myung Kyum
AU - Jung, Hee Young
N1 - Publisher Copyright:
© 2017, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2018/4/1
Y1 - 2018/4/1
N2 - The taxonomic position of bacterial strain, designated 15J9-4T, recovered from a beach soil sample on Jeju Island, South Korea, was established using a polyphasic approach. Strain 15J9-4T was assigned to phylum Bacteroidetes within the family Cytophagaceae based on 16S rRNA gene similarities. The closest phylogenetic relatives with validly published names were Spirosoma panaciterrae Gsoil 1519T (94.2% similarity) and Spirosoma luteolum 16F6ET (94.1%). Cells were rod-shaped, Gram-stain-negative, and non-motile. The isolate grew on NA, R2A, TSA, and LB agar. The temperature limits for growth were 10 and 30 °C with an optimum at 25 °C and the pH range was 7–8. Menaquinone MK-7 was the predominant respiratory quinone. The major cellular fatty acids comprised summed feature 3 (C16:1ω6c/C16:1ω7c, 30.2%), C16:1ω5c (22.2%), iso C15:0 (12.9%), and C16:0 (8.8%). Phosphatidylethanolamine was identified as the major polar lipid. The G+C content of the genomic DNA was 48.4 mol%. The results obtained from the polyphasic analyses allowed for the genotypic and phenotypic differentiation of strain 15J9-4T from recognized Spirosoma species. Therefore, the isolate is considered to represent a novel species in the genus Spirosoma, for which the name Spirosoma terrae sp. nov. is proposed. The type strain is 15J9-4T (= KCTC 52035T = JCM 31994T).
AB - The taxonomic position of bacterial strain, designated 15J9-4T, recovered from a beach soil sample on Jeju Island, South Korea, was established using a polyphasic approach. Strain 15J9-4T was assigned to phylum Bacteroidetes within the family Cytophagaceae based on 16S rRNA gene similarities. The closest phylogenetic relatives with validly published names were Spirosoma panaciterrae Gsoil 1519T (94.2% similarity) and Spirosoma luteolum 16F6ET (94.1%). Cells were rod-shaped, Gram-stain-negative, and non-motile. The isolate grew on NA, R2A, TSA, and LB agar. The temperature limits for growth were 10 and 30 °C with an optimum at 25 °C and the pH range was 7–8. Menaquinone MK-7 was the predominant respiratory quinone. The major cellular fatty acids comprised summed feature 3 (C16:1ω6c/C16:1ω7c, 30.2%), C16:1ω5c (22.2%), iso C15:0 (12.9%), and C16:0 (8.8%). Phosphatidylethanolamine was identified as the major polar lipid. The G+C content of the genomic DNA was 48.4 mol%. The results obtained from the polyphasic analyses allowed for the genotypic and phenotypic differentiation of strain 15J9-4T from recognized Spirosoma species. Therefore, the isolate is considered to represent a novel species in the genus Spirosoma, for which the name Spirosoma terrae sp. nov. is proposed. The type strain is 15J9-4T (= KCTC 52035T = JCM 31994T).
UR - http://www.scopus.com/inward/record.url?scp=85037672955&partnerID=8YFLogxK
U2 - 10.1007/s00284-017-1408-6
DO - 10.1007/s00284-017-1408-6
M3 - Article
C2 - 29209820
AN - SCOPUS:85037672955
SN - 0343-8651
VL - 75
SP - 492
EP - 498
JO - Current Microbiology
JF - Current Microbiology
IS - 4
ER -