Stable transcriptional status in the apoptotic erythroid genome

Sanggyu Lee; Junmo Hwang; Jodie Ulaszek; Yeong C. Kim; Hui Dong; Hyung Soo Kim; Ji Woong Seok; Bo Kyung Suh; So Jeong Yim; Debra Johnson; Nong Hoon Choe; Kyu Tae Chang; Zae Young Ryoo; Charles C. Tseng; Amittha Wickrema; San Ming Wang

doi:10.1016/j.bbrc.2007.05.168

Stable transcriptional status in the apoptotic erythroid genome

Sanggyu Lee, Junmo Hwang, Jodie Ulaszek, Yeong C. Kim, Hui Dong, Hyung Soo Kim, Ji Woong Seok, Bo Kyung Suh, So Jeong Yim, Debra Johnson, Nong Hoon Choe, Kyu Tae Chang, Zae Young Ryoo, Charles C. Tseng, Amittha Wickrema, San Ming Wang

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

When a cell is destined for apoptosis, will its genome reprogram its transcriptional machinery to overcome the life-threatening challenge? To address this issue, we performed a genome-wide transcriptome analysis in EPO (erythropoietin) deprivation-induced apoptotic erythroid cells using the SAGE method. The results show that the transcript contents for the majority of the genes remain unchanged in the apoptotic cells, including the apoptotic genes and the heat shock genes. Of the small number of genes with an altered expression, they are mainly associated with cellular structure. Our study reveals that there is no genetic reprogramming for the transcriptional machinery in the apoptotic genome. Apoptosis, as defined by programmed cell death, is not a crisis but a peaceful physiological process.

Original language	English
Pages (from-to)	556-562
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	359
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2007.05.168
State	Published - 3 Aug 2007

Keywords

Apoptosis
Erythroid
Gene expression
Genome
SAGE
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10.1016/j.bbrc.2007.05.168

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Lee, S., Hwang, J., Ulaszek, J., Kim, Y. C., Dong, H., Kim, H. S., Seok, J. W., Suh, B. K., Yim, S. J., Johnson, D., Choe, N. H., Chang, K. T., Ryoo, Z. Y., Tseng, C. C., Wickrema, A., & Wang, S. M. (2007). Stable transcriptional status in the apoptotic erythroid genome. Biochemical and Biophysical Research Communications, 359(3), 556-562. https://doi.org/10.1016/j.bbrc.2007.05.168

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title = "Stable transcriptional status in the apoptotic erythroid genome",

abstract = "When a cell is destined for apoptosis, will its genome reprogram its transcriptional machinery to overcome the life-threatening challenge? To address this issue, we performed a genome-wide transcriptome analysis in EPO (erythropoietin) deprivation-induced apoptotic erythroid cells using the SAGE method. The results show that the transcript contents for the majority of the genes remain unchanged in the apoptotic cells, including the apoptotic genes and the heat shock genes. Of the small number of genes with an altered expression, they are mainly associated with cellular structure. Our study reveals that there is no genetic reprogramming for the transcriptional machinery in the apoptotic genome. Apoptosis, as defined by programmed cell death, is not a crisis but a peaceful physiological process.",

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author = "Sanggyu Lee and Junmo Hwang and Jodie Ulaszek and Kim, {Yeong C.} and Hui Dong and Kim, {Hyung Soo} and Seok, {Ji Woong} and Suh, {Bo Kyung} and Yim, {So Jeong} and Debra Johnson and Choe, {Nong Hoon} and Chang, {Kyu Tae} and Ryoo, {Zae Young} and Tseng, {Charles C.} and Amittha Wickrema and Wang, {San Ming}",

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doi = "10.1016/j.bbrc.2007.05.168",

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AU - Lee, Sanggyu

AU - Hwang, Junmo

AU - Ulaszek, Jodie

AU - Kim, Yeong C.

AU - Dong, Hui

AU - Kim, Hyung Soo

AU - Seok, Ji Woong

AU - Suh, Bo Kyung

AU - Yim, So Jeong

AU - Johnson, Debra

AU - Choe, Nong Hoon

AU - Chang, Kyu Tae

AU - Ryoo, Zae Young

AU - Tseng, Charles C.

AU - Wickrema, Amittha

AU - Wang, San Ming

PY - 2007/8/3

Y1 - 2007/8/3

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AB - When a cell is destined for apoptosis, will its genome reprogram its transcriptional machinery to overcome the life-threatening challenge? To address this issue, we performed a genome-wide transcriptome analysis in EPO (erythropoietin) deprivation-induced apoptotic erythroid cells using the SAGE method. The results show that the transcript contents for the majority of the genes remain unchanged in the apoptotic cells, including the apoptotic genes and the heat shock genes. Of the small number of genes with an altered expression, they are mainly associated with cellular structure. Our study reveals that there is no genetic reprogramming for the transcriptional machinery in the apoptotic genome. Apoptosis, as defined by programmed cell death, is not a crisis but a peaceful physiological process.

KW - Apoptosis

KW - Erythroid

KW - Gene expression

KW - Genome

KW - SAGE

KW - Transcription

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SP - 556

EP - 562

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Stable transcriptional status in the apoptotic erythroid genome

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