TY - JOUR
T1 - Sterols isolated from Nuruk (Rhizopus oryzae KSD-815) inhibit the migration of cancer cells
AU - Lee, Dae Young
AU - Lee, Sang Jin
AU - Kwak, Ho Young
AU - Lakoon-Jung,
AU - Heo, Jieun
AU - Hong, Sungyoul
AU - Kim, Gye Won
AU - Baek, Nam In
PY - 2009/11
Y1 - 2009/11
N2 - An activity-guided fractionation method was used to isolate anticancer components from Nuruk (Rhizopus oryzae KSD-815:KSD-815). Dried powder of KSD-815 was extracted with 80% methanol and partitioned successively using nhexane, ethyl acetate, n-butanol, and water. The n-hexane and n-butanol fractions showed a strong antimigratory effect on human cancer cells. Both of these fractions were subjected to separation and purification procedures using silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies to afford four purified compounds. These were identified as ergosterol peroxide (1), stigmast-5-en-3β,7β-diol (2), ergosta-7,22-dien-3β,5α, 6β,9α-tetraol (3), and daucosterol (4), respectively, by spectroscopic methods such as nuclear magnetic resonance spectrometry, mass spectrometry, and infrared spectroscopy, and comparison with those in the literature. Compounds 1-4 were isolated from KSD-815 for the first time. Compounds 1 and 4 inhibited the migration of MDA-MB-231 cells at concentrations lower than 20 μM.
AB - An activity-guided fractionation method was used to isolate anticancer components from Nuruk (Rhizopus oryzae KSD-815:KSD-815). Dried powder of KSD-815 was extracted with 80% methanol and partitioned successively using nhexane, ethyl acetate, n-butanol, and water. The n-hexane and n-butanol fractions showed a strong antimigratory effect on human cancer cells. Both of these fractions were subjected to separation and purification procedures using silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies to afford four purified compounds. These were identified as ergosterol peroxide (1), stigmast-5-en-3β,7β-diol (2), ergosta-7,22-dien-3β,5α, 6β,9α-tetraol (3), and daucosterol (4), respectively, by spectroscopic methods such as nuclear magnetic resonance spectrometry, mass spectrometry, and infrared spectroscopy, and comparison with those in the literature. Compounds 1-4 were isolated from KSD-815 for the first time. Compounds 1 and 4 inhibited the migration of MDA-MB-231 cells at concentrations lower than 20 μM.
KW - Antimigratory effect
KW - MDA-MB-231
KW - Nuruk
KW - Rhizopus oryzae KSD-815
KW - SK-HEP-1
KW - Sterol
UR - http://www.scopus.com/inward/record.url?scp=71549159766&partnerID=8YFLogxK
U2 - 10.4014/jmb.0902.0072
DO - 10.4014/jmb.0902.0072
M3 - Article
C2 - 19996683
AN - SCOPUS:71549159766
SN - 1017-7825
VL - 19
SP - 1328
EP - 1332
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
IS - 11
ER -
