Stimulatory effect of nano-sized calcium metaphosphate particles on proliferation and osteoblastic differentiation of human bone marrow mesenchymal stem cells

Sun Young Lee, Min Jung Son, Gilson Khang, Youngsuk Son, Chang Kuk You, Suk Young Kim, Hong In Shin, Eui Kyun Park, Shin Yoon Kim

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Recently, nanomaterials have received considerable attention because of their potential applications in the biomedical field. In the present study, we investigated the effects of nano-sized calcium metaphosphate (CMP) particles (50 nm) compared with micro-sized CMP particles (200-500 nm and 10 μm) on the proliferation and osteoblastic differentiation of human bone marrow stem cells (BMSCs). BMSCs were challenged with CMP particles with different sizes for 3, 5, and 7 days. An analysis of the proliferation revealed that the nano-sized CMP particles (50 nm) stimulated the proliferation of BMSCs up to 27.79% compared to the untreated control. This stimulatory effect of the nano-sized CMP particle was dose-dependent. CMP particles appeared to adhere on the surface of BMSCs but this did not cause distinguishable morphological changes. Moreover, all CMP particles (50 nm to 10 μm) were capable of stimulating an osteoblastic differentiation of BMSCs as accessed by alkaline phosphatase (ALP) and von Kossa stainings. Further molecular analysis revealed that all the CMP particles induced an expression of osteoblast-related genes such as osteocalcin (OC) and collagen I (Col I). Taken together, our data demonstrate that nano-sized CMP particles have the potential to stimulate the proliferation and osteoblastic differentiation of BMSCs.

Original languageEnglish
Pages (from-to)1177-1180
Number of pages4
JournalKey Engineering Materials
Volume361-363 II
StatePublished - 2008

Keywords

  • Calcium phosphate ceramic
  • Mesenchymal stem cells
  • Nanoparticle
  • Osteoblastic differentiation
  • Proliferation

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